Evidence map›Paper›PMID 36555130›Full record

ArticleInternational journal of molecular sciences2022

Slight Variations in the Sequence Downstream of the Polyadenylation Signal Significantly Increase Transgene Expression in HEK293T and CHO Cells.

Evgeniya S Omelina, Anna E Letiagina, Lidiya V Boldyreva, Anna A Ogienko, Yuliya A Galimova, Lyubov A Yarinich, Alexey V Pindyurin, Evgeniya N Andreyeva

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Expression Pattern of the AB1-Gal4 Driver inInternational journal of molecular sciences · 2025
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Evgeniya S OmelinaDepartment of Regulation of Genetic Processes, Institute of Molecular and Cellular Biology, SB RAS, 630090 Novosibirsk, Russia.
Anna E LetiaginaDepartment of Regulation of Genetic Processes, Institute of Molecular and Cellular Biology, SB RAS, 630090 Novosibirsk, Russia.
Lidiya V BoldyrevaDepartment of Regulation of Genetic Processes, Institute of Molecular and Cellular Biology, SB RAS, 630090 Novosibirsk, Russia.ORCID 0000-0003-0716-4545
Anna A OgienkoDepartment of Regulation of Genetic Processes, Institute of Molecular and Cellular Biology, SB RAS, 630090 Novosibirsk, Russia.ORCID 0000-0002-0896-1899
Yuliya A GalimovaDepartment of Regulation of Genetic Processes, Institute of Molecular and Cellular Biology, SB RAS, 630090 Novosibirsk, Russia.
Lyubov A YarinichDepartment of Regulation of Genetic Processes, Institute of Molecular and Cellular Biology, SB RAS, 630090 Novosibirsk, Russia.ORCID 0000-0003-0469-0371
Alexey V PindyurinDepartment of Regulation of Genetic Processes, Institute of Molecular and Cellular Biology, SB RAS, 630090 Novosibirsk, Russia.ORCID 0000-0001-6959-0641
Evgeniya N AndreyevaDepartment of Regulation of Genetic Processes, Institute of Molecular and Cellular Biology, SB RAS, 630090 Novosibirsk, Russia.

Funding

Ministry of Science and Higher Education of the Russian Federation Agreement № 075-15-2021-1086, contract № RF----193021X0015, 15.ИП.21.0015
6 · The paper itself

Abstract

Compared to transcription initiation, much less is known about transcription termination. In particular, large-scale mutagenesis studies have, so far, primarily concentrated on promoter and enhancer, but not terminator sequences. Here, we used a massively parallel reporter assay (MPRA) to systematically analyze the influence of short (8 bp) sequence variants (mutations) located downstream of the polyadenylation signal (PAS) on the steady-state mRNA level of the upstream gene, employing an

Indexed as

PolyadenylationTranscription, GeneticAnimalsCHO CellsCricetinaeCricetulusHEK293 CellsHumansRNA, MessengerTransgenesRNA, Messengerbarcodegene expression regulationmassively parallel reporter assayMPRAnext-generation sequencingNGStranscription termination

Identifiers

PMID36555130
PMCPMC9779314

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.