ArticleInternational journal of environmental research and public health2022
Chemical Composition, Antimicrobial Activity, and Withdrawal Period of Essential Oil-Based Pharmaceutical Formulation in Bovine Mastitis Treatment.
Article in International journal of environmental research and public health, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it, 17 citations in OpenAlex.
- Pharmaco-toxicological aspects of thymol in veterinary medicine. A systematic review.Frontiers in veterinary science · 2025Pooled it
- Energy-sparing effect of oregano essential oil-lauric acid complex in broilers: Insights from serum metabolomics and 16S rRNA sequencing.Poultry science · 2026Article
- In Vitro Antimicrobial Potential of Medicinal Plant Extracts and Their Combinations Against Mastitis-Causing Bacteria in Dairy Cows.Molecules (Basel, Switzerland) · 2026Article
- Antibiotic Residues in Milk as a Consequence of Mastitis Treatment: Balancing Animal Welfare and One Health Risks.Veterinary sciences · 2025Review
- Clinical Evidence on Expansion of Essential Oil-Based Formulation's Pharmacological Activity in Bovine Mastitis Treatment: Antifungal Potential as Added Value.Antibiotics (Basel, Switzerland) · 2024Article
- Bovine mastitis, a worldwide impact disease: Prevalence, antimicrobial resistance, and viable alternative approaches.Veterinary and animal science · 2023Review
- Integration of Network Pharmacology and Molecular Docking to Analyse the Mechanism of Action of Oregano Essential Oil in the Treatment of Bovine Mastitis.Veterinary sciences · 2023Article
- Plant Essential Oils as a Tool in the Control of Bovine Mastitis: An Update.Molecules (Basel, Switzerland) · 2023Review
- Alternatives to Antimicrobial Treatment in Bovine Mastitis Therapy: A Review.Antibiotics (Basel, Switzerland) · 2023Review
- Intramammary Ethno-Veterinary Formulation in Bovine Mastitis Treatment for Optimization of Antibiotic Use.Pathogens (Basel, Switzerland) · 2023Article
- Pharmacoeconomic Analysis of the Different Therapeutic Approaches in Control of Bovine Mastitis: Phytotherapy and Antimicrobial Treatment.Antibiotics (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Due to the emergence of antibiotic-resistant bacteria, the risk it represents to public health, and the possible consequences for animal health and welfare, there is an increasing focus on reducing antimicrobial usage (AMU) in animal husbandry. Therefore, a great interest in developing alternatives to AMU in livestock production is present worldwide. Recently, essential oils (EOs) have gained great attention as promising possibilities for the replacement of antibiotics. The current study aimed to test the potential of using a novel EO-based pharmaceutical formulation (Phyto-Bomat) in bovine mastitis treatment. The antibacterial activity was performed using the microdilution technique. Lactating dairy cows were treated with 15 mL of Phyto-Bomat in the inflamed quarter for 5 consecutive days in order to analyze blood and milk samples for thymol and carvacrol residues using gas chromatography and mass spectrometry (GC-MS). Antimicrobial activity expressed as the minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) indicates that this formulation has the highest activity against Gram-positive strains. The dominant compounds in Phyto-Bomat were thymol and carvacrol, at 12.58 ± 1.23 mg/mL and 23.11 ± 2.31 mg/mL, respectively. The quantification of these two compounds in evaluated biological samples showed that 24 h after administration the concentration of thymol and carvacrol in milk samples was at the same level as before application. On the other hand, thymol and carvacrol were detectable in plasma samples even after 24 h post-treatment, with values ranging from 0.15-0.38 and 0.21-0.66 µg/mL, respectively. The tested formulation showed encouraging results of antibacterial activity against bovine mastitis pathogens, as well as the withdrawal period of dominant compounds, which implies that further testing regarding the bacteriological and clinical cure rates in clinical settings is needed.
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Registered trials
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