Evidence map›Paper›PMID 36551703›Full record

ArticleCancers2022

Circ_RNF13 Regulates the Stemness and Chemosensitivity of Colorectal Cancer by Transcriptional Regulation of DDX27 Mediated by TRIM24 Stabilization.

Yihang Guo, Gui Hu, Buning Tian, Min Ma, Fei Long, Miao Chen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancers, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Identification of Circular RNAs as Biomarker Candidates in Lung Cancer Treatment.Asian Pacific journal of cancer prevention : APJCP · 2024
    Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Yihang GuoDepartment of Gastrointestinal Surgery, The Third XiangYa Hospital, Central South University, Changsha 410013, China.
Gui HuDepartment of Gastrointestinal Surgery, The Third XiangYa Hospital, Central South University, Changsha 410013, China.
Buning TianDepartment of Gastrointestinal Surgery, The Third XiangYa Hospital, Central South University, Changsha 410013, China.
Min MaDepartment of Gastrointestinal Surgery, The Third XiangYa Hospital, Central South University, Changsha 410013, China.
Fei LongDepartment of Gastrointestinal Surgery, The Third XiangYa Hospital, Central South University, Changsha 410013, China.
Miao ChenDepartment of Gastrointestinal Surgery, The Third XiangYa Hospital, Central South University, Changsha 410013, China.
Central South University · CN

Funding

FACTORS THAT MODIFY INSULIN ACTIONR01DK002001 · NIDDK · MEDICAL UNIVERSITY OF SOUTH CAROLINA · PI BUSE, MARIA G · 1986 to 2012
$3.1M
Factors that modify insulin actionR56DK002001 · NIDDK · MEDICAL UNIVERSITY OF SOUTH CAROLINA · PI BUSE, MARIA G · 2008 to 2008
$199k
NIDDK NIH HHS R01 DK002001NIDDK NIH HHS R56 DK002001
6 · The paper itself

Abstract

backgroundColorectal cancer (CRC) is one of the most commonly diagnosed cancers with high incidence and poor prognosis worldwide. Circ_RNF13 is upregulated in CRC; however, the biological roles and downstream signaling of circ_RNF13 remain undefined.

methodsThe characterization of circ_RNF13 was determined by Sanger sequencing, qRT-PCR, subcellular fractionation assay, and RNA FISH. Western blot analysis and qRT-PCR were employed to detect the expression of the key molecules and stemness markers in CRC tumor samples and cells. The stem-like activities of CRC cells were assessed by sphere formation assay, flow cytometry, and immunofluorescence (IF). Cell viability was monitored by CCK-8 assay. The chemosensitivity of CRC cells was assessed by colony formation and cell apoptosis assays. Bioinformatics analysis, RIP assay, RNA pull-down assay, and FISH/IF staining were used to detect the association between circ_RNF13 and TRIM24. The transcriptional regulation of DDX27 was investigated by ChIP assay, and the post-translational regulation of TRIM24 was detected by Co-IP. The in vitro findings were verified in a xenograft model.

resultscirc_RNF13 and DDX27 were elevated in CRC tumor samples and cells. Knockdown of circ_RNF13 or DDX27 inhibited stemness and increased chemosensitivity in CRC cells. Mechanistically, circ_RNF13 regulated DDX27 expression via TRIM24-mediated transcriptional regulation, and circ_RNF13 stabilized TRIM24 via suppressing FBXW7-mediated TRIM24 degradation. In vivo studies revealed that the knockdown of circ_RNF13 impaired stemness and enhanced the chemosensitivity of CRC in the xenograft model.

conclusioncirc_RNF13 regulated the stemness and chemosensitivity of CRC by transcriptional regulation of DDX27 mediated by TRIM24 stabilization.

Indexed as

circ_RNF13colorectal cancerDDX27TRIM24

Identifiers

PMID36551703
PMCPMC9776557
OpenAlexW4312140847

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.