Evidence map›Paper›PMID 36551353›Full record

ArticleAntibiotics (Basel, Switzerland)2022

Detecting Carbapenemases in Animal and Food Samples by Droplet Digital PCR.

Maria Carelli, Francesca Griggio, Marina Mingoia, Cristiana Garofalo, Vesna Milanović, Nicola Pozzato, Francesca Leoni, Laura Veschetti, Giovanni Malerba, Angela Sandri and 4 more

Open access · goldAbstract read
In one paragraph

Article in Antibiotics (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.4field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 1 country.

Maria CarelliDepartment of Diagnostics and Public Health, University of Verona, 37134 Verona, Italy.
Francesca GriggioCentro Piattaforme Tecnologiche, University of Verona, 37134 Verona, Italy.
Marina MingoiaDepartment of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60121 Ancona, Italy.ORCID 0000-0001-8225-7843
Cristiana GarofaloDepartment of Agricultural, Food and Environmental Sciences, Polytechnic University of Marche, 60121 Ancona, Italy.
Vesna MilanovićDepartment of Agricultural, Food and Environmental Sciences, Polytechnic University of Marche, 60121 Ancona, Italy.ORCID 0000-0001-8548-366X
Nicola PozzatoLaboratorio di Diagnostica Clinica e Sierologia di Piano, Istituto Zooprofilattico Sperimentale delle Venezie, 37060 Buttapietra, Italy.ORCID 0000-0002-2510-1241
Francesca LeoniLaboratorio Nazionale di Riferimento (LNR) per il Controllo delle Contaminazioni Batteriche dei Molluschi Bivalvi, Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche "Togo Rosati", 60121 Ancona, Italy.
Laura VeschettiDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, 37134 Verona, Italy.ORCID 0000-0002-0413-6031
Giovanni MalerbaDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, 37134 Verona, Italy.ORCID 0000-0001-8705-8560
Angela SandriDepartment of Diagnostics and Public Health, University of Verona, 37134 Verona, Italy.ORCID 0000-0002-5107-0960
Cristina PatuzzoDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, 37134 Verona, Italy.ORCID 0000-0001-8678-9337
Serena SimoniDepartment of Life and Environmental Sciences, Polytechnic University of Marche, 60121 Ancona, Italy.
Maria M LleoDepartment of Diagnostics and Public Health, University of Verona, 37134 Verona, Italy.
Carla VignaroliDepartment of Life and Environmental Sciences, Polytechnic University of Marche, 60121 Ancona, Italy.
University of Verona · ITMarche Polytechnic University · ITIstituto Zooprofilattico Sperimentale delle Venezie · ITIstituto Zooprofilattico Sperimentale dell'Umbria e delle Marche · IT

Funding

Fondazione Cariverona 9210
6 · The paper itself

Abstract

backgroundThe presence of carbapenemase-producing bacteria (CPB) in animal hosts and along the food chain may result in the development of reservoirs for human infections. Several CPB strains isolated from animals have been reported, suggesting that transmission and dissemination of the corresponding genes between humans and animals may occur. Animal and food samples have complex backgrounds that hinder the detection of CPB present in low concentrations by standard detection procedures.

methodsWe evaluated the possibility of detecting

resultsThe proposed ddPCR managed to detect the target genes, also in samples resulting negative to standard methods. While the presence of

conclusionA specific and sensitive method such as ddPCR could be suitable to evaluate the current veterinarian and environmental situation and to assess the dynamic transmission and persistence of CPB between animals and humans and vice versa.

Indexed as

animal samplescarbapenemasesdroplet digital PCRfood samplesmultidrug resistance

Identifiers

PMID36551353
PMCPMC9774140
OpenAlexW4310039589

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.