Evidence map›Paper›PMID 36551031›Full record

ArticleBiosensors2022

Bare Eye Detection of Bacterial Enzymes of

Qasim Alhusaini, Walter Sebastian Scheld, Zhiyuan Jia, Dipankar Das, Faria Afzal, Mareike Müller, Holger Schönherr

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Qasim AlhusainiPhysical Chemistry I and Research Center of Micro and Nanochemistry and (Bio)Technology (Cμ), Department of Chemistry and Biology, School of Science and Technology, University of Siegen, Adolf-Reichwein-Str. 2, 57076 Siegen, Germany.ORCID 0000-0002-6743-0115
Walter Sebastian ScheldPhysical Chemistry I and Research Center of Micro and Nanochemistry and (Bio)Technology (Cμ), Department of Chemistry and Biology, School of Science and Technology, University of Siegen, Adolf-Reichwein-Str. 2, 57076 Siegen, Germany.ORCID 0000-0003-0692-2404
Zhiyuan JiaPhysical Chemistry I and Research Center of Micro and Nanochemistry and (Bio)Technology (Cμ), Department of Chemistry and Biology, School of Science and Technology, University of Siegen, Adolf-Reichwein-Str. 2, 57076 Siegen, Germany.ORCID 0000-0002-9072-8278
Dipankar DasPhysical Chemistry I and Research Center of Micro and Nanochemistry and (Bio)Technology (Cμ), Department of Chemistry and Biology, School of Science and Technology, University of Siegen, Adolf-Reichwein-Str. 2, 57076 Siegen, Germany.ORCID 0000-0001-9298-6871
Faria AfzalPhysical Chemistry I and Research Center of Micro and Nanochemistry and (Bio)Technology (Cμ), Department of Chemistry and Biology, School of Science and Technology, University of Siegen, Adolf-Reichwein-Str. 2, 57076 Siegen, Germany.
Mareike MüllerPhysical Chemistry I and Research Center of Micro and Nanochemistry and (Bio)Technology (Cμ), Department of Chemistry and Biology, School of Science and Technology, University of Siegen, Adolf-Reichwein-Str. 2, 57076 Siegen, Germany.
Holger SchönherrPhysical Chemistry I and Research Center of Micro and Nanochemistry and (Bio)Technology (Cμ), Department of Chemistry and Biology, School of Science and Technology, University of Siegen, Adolf-Reichwein-Str. 2, 57076 Siegen, Germany.ORCID 0000-0002-5836-5569

Funding

Alexander von Humboldt Foundation postdoctoral fellowship to DDDAAD Ph.D. stipend to QAMax-Buchner-Forschungsstiftung-Dechema MBFSt-Kennziffer: 3671University of Siegen n/a
6 · The paper itself

Abstract

The fabrication, characterization and application of a nanoporous Silicon Rugate Filter (pSiRF) loaded with an enzymatically degradable polymer is reported as a bare eye detection optical sensor for enzymes of pathogenic bacteria, which is devoid of any dyes. The nanopores of pSiRF were filled with poly(lactic acid) (PLA), which, upon enzymatic degradation, resulted in a change in the effective refractive index of the pSiRF film, leading to a readily discernible color change of the sensor. The shifts in the characteristic fringe patterns before and after the enzymatic reaction were analyzed quantitatively by Reflectometric Interference Spectroscopy (RIfS) to estimate the apparent kinetics and its dependence on enzyme concentration. A clear color change from green to blue was observed by the bare eye after PLA degradation by proteinase K. Moreover, the color change was further confirmed in measurements in bacterial suspensions of the pathogen

Indexed as

NanoporesPolymersPolyestersPseudomonas aeruginosaSiliconPolyestersPolymersSiliconbacteria detectionbacterial enzymebiodegradable polymernanoporous silicon rugate filterpoly(lactic acid)Pseudomonas aeruginosa

Identifiers

PMID36551031
PMCPMC9776340

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.