ArticleBioengineering (Basel, Switzerland)2022
Three-Dimensional Bioprinting with Alginate by Freeform Reversible Embedding of Suspended Hydrogels with Tunable Physical Properties and Cell Proliferation.
Article in Bioengineering (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Revolutionizing vision using corneal 3D printing.Trends in biotechnology · 2026Review
- Handheld Bioprinters in Skin Regeneration: Current Landscape, Clinical Promise, and the Road Ahead.Biomedicines · 2026Review
- Modified Polysaccharides: Potential Biomaterials for Bioprinting.Journal of functional biomaterials · 2025Review
- Proximity Labeling and SILAC-Based Proteomic Approach Identifies Proteins at the Interface of Homotypic and Heterotypic Cancer Cell Interactions.Molecular & cellular proteomics : MCP · 2025Article
- Extrusion bioprinting: meeting the promise of human tissue biofabrication?Progress in biomedical engineering (Bristol, England) · 2025Review
- Gel-Based Suspension Medium Used in 3D Bioprinting for Constructing Tissue/Organ Analogs.Gels (Basel, Switzerland) · 2024Review
- Diffusion-Based 3D Bioprinting Strategies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Nanocrystalline Cellulose as a Versatile Engineering Material for Extrusion-Based Bioprinting.Pharmaceutics · 2023Article
- Biomaterials / bioinks and extrusion bioprinting.Bioactive materials · 2023Review
- Cell-Tissue Interaction: The Biomimetic Approach to Design Tissue Engineered Biomaterials.Bioengineering (Basel, Switzerland) · 2023Review
- Advances in 3D Bioprinting: Techniques, Applications, and Future Directions for Cardiac Tissue Engineering.Bioengineering (Basel, Switzerland) · 2023Review
- Optimization of Freeform Reversible Embedding of Suspended Hydrogel Microspheres for Substantially Improved Three-Dimensional Bioprinting Capabilities.Tissue engineering. Part C, Methods · 2023Article
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
Abstract
Extrusion-based three-dimensional (3D) bioprinting is an emerging technology that allows for rapid bio-fabrication of scaffolds with live cells. Alginate is a soft biomaterial that has been studied extensively as a bio-ink to support cell growth in 3D constructs. However, native alginate is a bio-inert material that requires modifications to allow for cell adhesion and cell growth. Cells grown in modified alginates with the RGD (arginine-glycine-aspartate) motif, a naturally existing tripeptide sequence that is crucial to cell adhesion and proliferation, demonstrate enhanced cell adhesion, spreading, and differentiation. Recently, the bioprinting technique using freeform reversible embedding of suspended hydrogels (FRESH) has revolutionized 3D bioprinting, enabling the use of soft bio-inks that would otherwise collapse in air. However, the printability of RGD-modified alginates using the FRESH technique has not been evaluated. The associated physical properties and bioactivity of 3D bio-printed alginates after RGD modification remains unclear. In this study, we characterized the physical properties, printability, and cellular proliferation of native and RGD-modified alginate after extrusion-based 3D bioprinting in FRESH. We demonstrated tunable physical properties of native and RGD-modified alginates after FRESH 3D bioprinting. Sodium alginate with RGD modification, especially at a high concentration, was associated with greatly improved cell viability and integrin clustering, which further enhanced cell proliferation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.