Evidence map›Paper›PMID 36550401›Full record

ArticleBMC cardiovascular disorders2022

Vitamin C alleviates LPS-induced myocardial injury by inhibiting pyroptosis via the ROS-AKT/mTOR signalling pathway.

Pu Zhang, Meirong Zang, Zhenzhen Sang, Yunxia Wei, Yan Yan, Xiaohua Bian, Shimin Dong

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cardiovascular disorders, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Review
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  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Pu ZhangDepartment of Emergency, The Third Hospital of Hebei Medical University, Zi-Qiang Road No. 139, Shijiazhuang, 050051, Hebei, China.
Meirong ZangDepartment of Haematology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
Zhenzhen SangDepartment of Emergency, The Third Hospital of Hebei Medical University, Zi-Qiang Road No. 139, Shijiazhuang, 050051, Hebei, China.
Yunxia WeiDepartment of Emergency, The Third Hospital of Hebei Medical University, Zi-Qiang Road No. 139, Shijiazhuang, 050051, Hebei, China.
Yan YanDepartment of Emergency, The Third Hospital of Hebei Medical University, Zi-Qiang Road No. 139, Shijiazhuang, 050051, Hebei, China.
Xiaohua BianDepartment of Emergency, The Third Hospital of Hebei Medical University, Zi-Qiang Road No. 139, Shijiazhuang, 050051, Hebei, China.
Shimin DongDepartment of Emergency, The Third Hospital of Hebei Medical University, Zi-Qiang Road No. 139, Shijiazhuang, 050051, Hebei, China. woshiyisheng2014@126.com.
Hebei Medical University · CNThird Hospital of Hebei Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe efficacy of vitamin C in sepsis remains controversial. Whether vitamin C can alleviate lipopolysaccharide (LPS)-induced myocardial injury by inhibiting pyroptosis has not been studied. This study aimed to evaluate the effects of vitamin C on LPS-induced myocardial injury in vitro.

methodsH9C2 cells were treated with indicated concentrations of LPS, and the cell viability was then assessed by CCK-8 assay. The levels of lactate dehydrogenase (LDH), CK-MB, IL-18 and IL-1β were examined by enzyme-linked immunosorbent assay (ELISA). The levels of intracellular reactive oxygen species (ROS) were measured using the fluorescent probe dichlorodihydrofluorescein diacetate (DCFH-DA). Western blot assays were conducted to determine the levels of the ROS-associated protein nicotinamide adenine dinucleotide phosphate oxidase 4 (Nox4) and pyroptosis-associated proteins, such as NOD-like receptor (NLR) family pyrin domain containing 3 (NLRP3), caspase-1 and gasdermin D (GSDMD). The AKT inhibitor MK-2206 was then applied to explore the signalling pathway. Finally, H9C2 cells were divided into the control group, LPS group, vitamin C + LPS group, and N-acetyl-L-cysteine (NAC) + LPS group. The intracellular ROS, levels of associated proteins, cell viability, and release of LDH, CK-MB, IL-18 and IL-1β were examined.

resultsLPS decreased cell viability and induced ROS and pyroptosis in H9C2 cells in a dose-dependent manner. Moreover, LPS activated the AKT/mTOR pathway in H9C2 cells. The AKT inhibitor MK-2206 protected H9C2 cells from LPS-induced death by suppressing pyroptosis, without changing intracellular ROS level. Vitamin C significantly inhibited intracellular ROS and cell pyroptosis in LPS-treated H9C2 cells. Moreover, vitamin C suppressed the activation of the AKT/mTOR pathway.

conclusionsOur data suggest that vitamin C alleviates LPS-induced myocardial injury by inhibiting pyroptosis via the ROS-AKT/mTOR signalling pathway and thus provide novel insights into the prevention of sepsis-induced myocardial dysfunction.

Indexed as

LipopolysaccharidesSepsisAscorbic AcidHumansInterleukin-18NLR Family, Pyrin Domain-Containing 3 ProteinProto-Oncogene Proteins c-aktPyroptosisReactive Oxygen SpeciesTOR Serine-Threonine KinasesVitaminsAscorbic AcidInterleukin-18LipopolysaccharidesMTOR protein, humanNLR Family, Pyrin Domain-Containing 3 ProteinProto-Oncogene Proteins c-aktReactive Oxygen SpeciesTOR Serine-Threonine KinasesVitaminsMyocardial injuryPyroptosisReactive oxygen species (ROS)SepsisVitamin C

Identifiers

PMID36550401
PMCPMC9783737
OpenAlexW4312190859

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.