ArticleFrontiers in microbiology2022
A triton X-100 assisted PMAxx-qPCR assay for rapid assessment of infectious African swine fever virus.
Article in Frontiers in microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 13 citations in OpenAlex.
- Optimized Viability-ddPCR with Triton X-100 Enhancement for Selective Detection of LiveJournal of microbiology and biotechnology · 2026Article
- Innovations, Applications, and Future Trends in Veterinary Diagnostic Technologies.Transboundary and emerging diseases · 2026Review
- Rapid differentiation of viable and inactivated African swine fever virus by a viability quantitative PCR.Veterinary research · 2025Article
- Refining microbial biomarker identification in rumen microbiome studies: a viability PCR-based approach.Applied and environmental microbiology · 2025Article
- Measurement of the Infection and Integrity of Monkeypox Virus: A New Method Using PMAxx-ddPCR.International journal of molecular sciences · 2025Article
- A Triplex Propidium Monoazide (PMA) qPCR Assay Enables Rapid Discrimination of Live Porcine Reproductive and Respiratory Syndrome Viruses.Transboundary and emerging diseases · 2025Article
- Propidium Monoazide Integrated With qPCR Enables Rapid and Universal Detection of Infectious Porcine Reproductive and Respiratory Syndrome Viruses.Transboundary and emerging diseases · 2024Article
- Quickly assessing disinfection effectiveness to control the spread of African swine fever virus.Applied microbiology and biotechnology · 2023Article
- Application of propidium monoazide quantitative PCR to discriminate of infectious African swine fever viruses.Frontiers in microbiology · 2023Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introduction: African Swine Fever (ASF) is a highly infectious disease of pigs, caused by Methods: In this study, a Triton X-100 assisted PMAxx-qPCR method was developed for rapid assessment of infectious ASFV in samples. PMAxx, an improved version of propidium monoazide (PMA), can covalently cross-link with naked ASFV-DNA or DNA inside inactivated ASFV virions under assistance of 0.1% (v/v) TritonX-100, but not with ASFV-DNA inside live virions. Formation of PMAxx-DNA conjugates prevents PCR amplification, leaving only infectious virions to be detected. Under optimum conditions, the limit of detection of the PMAxx-qPCR assay was 2.32log Conclusion: The Triton X-100 assisted PMAxx-qPCR assay took less than 3 h from sample to result, offering an easier and faster way for assessing infectious ASFV in samples from places like pig farms and pork markets.
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Registered trials
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