ArticleRSC advances2022
2,5-Furandicarboxaldehyde as a bio-based crosslinking agent replacing glutaraldehyde for covalent enzyme immobilization.
Article in RSC advances, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Valorization of fish oil byproduct methyl esters for omega‑3 polyunsaturated fatty acids production via immobilized CALB lipase‑catalyzed hydrolysis.Bioprocess and biosystems engineering · 2026Article
- Scaling Up Is Not the Same but Bigger: Overcoming (Some) Limitations in Enzymatic Decarboxylations in Rotating Bed Reactors in Deep Eutectic Solvents.Organic process research & development · 2026Article
- Immobilization of crude cellulase from Aspergillus foetidus on agar xerogel and its application for carrot juice extraction.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Semi-interpenetrating polymer network ion-solvating membrane with enhanced conductivity and stability for potential application in alkaline water electrolysis.RSC advances · 2025Article
- A Comprehensive Guide to Enzyme Immobilization: All You Need to Know.Molecules (Basel, Switzerland) · 2025Review
- Latest Trends in Lipase-Catalyzed Synthesis of Ester Carbohydrate Surfactants: From Key Parameters to Opportunities and Future Development.International journal of molecular sciences · 2024Review
- Spectrophotometric Assay for the Detection of 2,5-Diformylfuran and Its Validation through Laccase-Mediated Oxidation of 5-Hydroxymethylfurfural.International journal of molecular sciences · 2023Article
- Engineering of PVA/PVP Hydrogels for Agricultural Applications.Gels (Basel, Switzerland) · 2023Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the quest for a bio-based and safer substitute for glutaraldehyde, we have investigated 2,5 diformylfuran (DFF) as bifunctional crosslinking agent for the covalent immobilization of glucoamylase on amino-functionalized methacrylic resins. Immobilization experiments and systematic comparison with glutaraldehyde at four different concentrations for the activation step showed that DFF leads to comparable enzymatic activities at all tested concentrations. Continuous flow experiment confirms a similar long term stability of the immobilized formulations obtained with the two crosslinkers. The NMR study of DFF in aqueous solution evidenced a much simpler behaviour as compared to glutaraldehyde, since no enolic forms can form and only a mono-hydrated form was observed. Unlike in the case of glutaraldehyde, DFF reacts covalently with the primary amino groups
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.