Evidence map›Paper›PMID 36544577›Full record

ArticleRSC chemical biology2022

Chemical synthesis of the EPF-family of plant cysteine-rich proteins and late-stage dye attachment by chemoselective amide-forming ligations.

Nandarapu Kumarswamyreddy, Ayami Nakagawa, Hitoshi Endo, Akie Shimotohno, Keiko U Torii, Jeffrey W Bode, Shunsuke Oishi

Open access · goldAbstract read
In one paragraph

Article in RSC chemical biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 4 countries.

Nandarapu KumarswamyreddyInstitute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University Chikusa Nagoya 464-8602 Japan oishi@itbm.nagoya-u.ac.jp.ORCID https://orcid.org/0000-0002-7640-1201
Ayami NakagawaInstitute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University Chikusa Nagoya 464-8602 Japan oishi@itbm.nagoya-u.ac.jp.ORCID https://orcid.org/0000-0001-7230-9451
Hitoshi EndoInstitute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University Chikusa Nagoya 464-8602 Japan oishi@itbm.nagoya-u.ac.jp.
Akie ShimotohnoInstitute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University Chikusa Nagoya 464-8602 Japan oishi@itbm.nagoya-u.ac.jp.
Keiko U ToriiInstitute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University Chikusa Nagoya 464-8602 Japan oishi@itbm.nagoya-u.ac.jp.ORCID https://orcid.org/0000-0002-6168-427X
Jeffrey W BodeInstitute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University Chikusa Nagoya 464-8602 Japan oishi@itbm.nagoya-u.ac.jp.ORCID https://orcid.org/0000-0001-8394-8910
Shunsuke OishiInstitute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University Chikusa Nagoya 464-8602 Japan oishi@itbm.nagoya-u.ac.jp.ORCID https://orcid.org/0000-0001-8807-9072
Nagoya University · JPETH Zurich · CHHoward Hughes Medical Institute · USIndian Institute of Technology Tirupati · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chemical protein synthesis can provide well-defined modified proteins. Herein, we report the chemical synthesis of plant-derived cysteine-rich secretory proteins and late-stage derivatization of the synthetic proteins. The syntheses were achieved with distinct chemoselective amide bond forming reactions - EPF2 by native chemical ligation (NCL), epidermal patterning factor (EPF) 1 by the α-ketoacid-hydroxylamine (KAHA) ligation, and fluorescent functionalization of their folded variants by potassium acyltrifluoroborate (KAT) ligation. The chemically synthesized EPFs exhibit bioactivity on stomatal development in

Identifiers

PMID36544577
PMCPMC9709926
OpenAlexW4312113744

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.