ArticleTurkish journal of pharmaceutical sciences2022
Whole Genome Sequencing of Antibiotic Resistant Genes in Isolates from Surfaces in a Science Laboratory.
Article in Turkish journal of pharmaceutical sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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1 citing paper in PubMed, 1 citations in OpenAlex.
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Objectives: Isolates obtained from laboratory surfaces were identified and characterized. Materials and Methods: Ten consecutive isolates were obtained from 30 sample surfaces of a University Science Laboratory in Edo State Nigeria in May, 2021. Swabs of surfaces from the laboratory were obtained aseptically. The sample swabs were streaked on MacConkey, eosin methylene blue, mannitol salt, and nutrient agar plates, respectively, and incubated appropriately. Distinct colonies were randomly obtained from culture plates and characterized phenotypically. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was used to analyze four isolates (40%) obtained by selection criteria. Susceptibility testing using antibiotics was performed for the identified isolates by Kirby-Bauer method for 15 antibiotics. Isolate characterization and identification of resistance determinants were determined using whole genome sequencing (WGS). Results: Microorganisms identified included Conclusion: Our study displays the dissemination of antibiotic resistance among isolates obtained from surface of a University Science Laboratory. To the best of our knowledge, we have reported the first genomic characterization of resistance to antibiotics in isolates obtained from surfaces of a University Science Laboratory in Nigeria.
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