Evidence map›Paper›PMID 36542310›Full record

SynthesisJournal of assisted reproduction and genetics2023

Vitrification versus slow freezing of human ovarian tissue: a systematic review and meta-analysis of histological outcomes.

Supriya Behl, Vidhu B Joshi, Nicholas B Larson, Maia C Young, Muhammad Bilal, David L Walker, Zaraq Khan, Candace F Granberg, Asma Chattha, Yulian Zhao

Abstract readMeta-AnalysisSystematic Review
PubMed Full text at PMC Publisher
In one paragraph

Synthesis in Journal of assisted reproduction and genetics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
  2. Pooled it
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Cryopreservation of Ovarian Cortex Tissue.Methods in molecular biology (Clifton, N.J.) · 2026
    Article
  12. Article
  13. Review
  14. Article
  15. Review
  16. Article
  17. Article
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Supriya Behl *Children's Research Center, Department of Pediatric and Adolescent Medicine, Mayo Clinic, 200 1st St SW, MN, 55905, Rochester, USA.
Vidhu B Joshi *Charles Widger School of Law, Villanova University, Villanova, PA, USA.
Nicholas B LarsonDepartment of Quantitative Health Sciences, Mayo Clinic, Rochester, MN, 55905, USA.
Maia C YoungMayo Clinic Alix School of Medicine, Rochester, MN, 55905, USA.
Muhammad BilalDivision of Pediatric and Adolescent Gynecology, Department of Pediatric and Adolescent Medicine, Mayo Clinic, 200 1st St SW, MN, 55905, Rochester, USA.
David L WalkerDivision of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Mayo Clinic, 200 1st St SW, MN, 55905, Rochester, USA.
Zaraq KhanDivision of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Mayo Clinic, 200 1st St SW, MN, 55905, Rochester, USA.
Candace F GranbergDepartment of Urology, Mayo Clinic, 200 1st St SW, MN, 55905, Rochester, USA.
Asma ChatthaDivision of Pediatric and Adolescent Gynecology, Department of Pediatric and Adolescent Medicine, Mayo Clinic, 200 1st St SW, MN, 55905, Rochester, USA.
Yulian ZhaoDivision of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Mayo Clinic, 200 1st St SW, MN, 55905, Rochester, USA. zhao.yulian@mayo.edu.ORCID http://orcid.org/0000-0002-4591-4207

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A systematic review and meta-analysis of pertinent literature published from 2006 to January 2022 were conducted to study and compare vitrification and slow freezing, the two prominent methods of ovarian tissue cryopreservation. The primary outcome measures for this study were (1) proportion of intact primordial follicles, (2) proportion of intact stromal cells, (3) proportion of DNA fragmentation in primordial follicles, and (4) mean primordial follicle density. This meta-analysis of 19 studies revealed a significantly greater proportion of intact stromal cells in vitrified tissue versus slow-frozen tissue. No significant differences upon pooled analyses were observed between the two cryopreservation methods with respect to the proportion of intact primordial follicles, proportion of DNA fragmentation, or mean primordial follicle density. Due to differences seen in stromal cell viability, vitrification may be a preferred option to preserve histology of tissue. However, more work should be done to compare the two freezing techniques with less heterogeneity caused by patients, samples, and protocols.

Indexed as

OvaryVitrificationCryopreservationFemaleFreezingHumansOvarian FollicleFertility preservationOvarian tissue cryopreservationSlow freezingVitrification

Identifiers

PMID36542310
PMCPMC10033773

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.