Evidence map›Paper›PMID 36542235›Full record

ArticleMolecular biology reports2023

Heterologous expression of quorum sensing transcriptional regulator LitR and its function in virulence-related gene regulation in foodborne pathogen Aeromonas hydrophila.

Jing Zhao, Yue Li, Yan Huang, Liming Jin, Yongbin Xu, Menghao Xu, Chunshan Quan, Ming Chen

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Article in Molecular biology reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 47% of its field
1 · What the graph read from it

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3 · Its place in the literature

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1 citing paper in PubMed, 3 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Jing ZhaoKey Laboratory of Biotechnology and Bioresources Utilization (Dalian Minzu University), Ministry of Education, Dalian, 116600, China.
Yue LiCollege of Bioengineering, Dalian Polytechnic University, Dalian, 116034, China.
Yan HuangCollege of Bioengineering, Dalian Polytechnic University, Dalian, 116034, China.
Liming JinKey Laboratory of Biotechnology and Bioresources Utilization (Dalian Minzu University), Ministry of Education, Dalian, 116600, China.
Yongbin XuKey Laboratory of Biotechnology and Bioresources Utilization (Dalian Minzu University), Ministry of Education, Dalian, 116600, China.
Menghao XuCollege of Life Science, Dalian Minzu University, Dalian, 116600, China.
Chunshan QuanKey Laboratory of Biotechnology and Bioresources Utilization (Dalian Minzu University), Ministry of Education, Dalian, 116600, China. mikyeken@dlnu.edu.cn.
Ming ChenCollege of Bioengineering, Dalian Polytechnic University, Dalian, 116034, China. chenming@dlpu.edu.cn.
Dalian Minzu University · CNDalian Polytechnic University · CNMinzu University of China · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAeromonas hydrophila is an important foodborne and zoonotic pathogen causing serious diseases. Hence, revealing the pathogenic mechanism of A. hydrophila will be of importance in the development of novel therapies. Aeromonas hydrophila litR was reported to be regulated by two quorum sensing (QS) pathways, indicating that it is involved in QS network regulation correlated with bacterial virulence. However, the function of LitR is currently not understood. Therefore, we aimed to reveal the potential regulatory mechanisms of LitR on virulence-related genes. METHODS AND

resultsIn this study, amino acid sequences analysis of LitR was conducted, providing bioinformatics evidence for its function as a potential transcriptional regulator. LitR protein was heterologous expressed, purified and its in-vitro multimeric forms were observed with gel filtration chromatography. The correlation between intracellular LitR expression level and cell density was analyzed with immunoblots. Regulation mechanisms of LitR on several important virulence-related factors were investigated with qRT-PCR, EMSA, DNase I footprinting and microscale thermophoresis binding assays, etc. Results showed that recombinant LitR protein aggregated mainly as dimer and hexamer in vitro. Intracellular expression level of LitR was positively correlated with cell density of A. hydrophila. Furthermore, LitR exhibited complicated regulation modes on virulence-related genes; it could directly bind to promoter regions of the hemolysin, serine protease and T6SS effector protein VgrG encoded genes. The promoter region of the hemolysin gene showed high binding affinity and mainly two binding sites for LitR. Different dissociation constants were obtained for LitR interaction with the hemolysin gene binding motifs I and II. Assays focusing on physiological characteristics of A. hydrophila prove that LitR positively regulated hemolytic and total extracellular protease activities.

conclusionsThis study investigated the function of LitR as a quorum sensing transcriptional regulator in regulation of virulence-related genes, which will help reveal the mechanisms of A. hydrophila pathogenicity. LitR could serve as a potential target for development of new antimicrobial agents from the perspective of QS regulation.

Indexed as

BiofilmsQuorum SensingAeromonas hydrophilaBacterial ProteinsGene Expression Regulation, BacterialHemolysin ProteinsRecombinant ProteinsVirulenceVirulence FactorsBacterial ProteinsHemolysin ProteinsRecombinant ProteinsVirulence FactorsAeromonas hydrophilaLitRQuorum sensingRegulation mechanismVirulence-related factor

Identifiers

PMID36542235
OpenAlexW4312067078

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.