Ligand-binding assay based on microfluidic chemotaxis of porphyrin receptors.

Bin Li, Kejiao Gao, Yurong Li, Yuansheng Li, Longyi Zhu, Xuanyu Fu, Xiyong Zhuo, Ying Wu, Ying Wan, Shengyuan Deng

Abstract read

In one paragraph

Article in Chemical science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Bin LiSchool of Environmental and Biological Engineering, Nanjing University of Science and Technology Nanjing 210094 P. R. China sydeng@njust.edu.cn.

Kejiao GaoSchool of Environmental and Biological Engineering, Nanjing University of Science and Technology Nanjing 210094 P. R. China sydeng@njust.edu.cn.

Yurong LiSchool of Environmental and Biological Engineering, Nanjing University of Science and Technology Nanjing 210094 P. R. China sydeng@njust.edu.cn.

Yuansheng LiSchool of Environmental and Biological Engineering, Nanjing University of Science and Technology Nanjing 210094 P. R. China sydeng@njust.edu.cn.

Longyi ZhuSchool of Chemistry and Chemical Engineering, Nanjing University of Science and Technology Nanjing 210094 P. R. China lyzhu@njust.edu.cn.ORCID https://orcid.org/0000-0002-0898-4804

Xuanyu FuSchool of Environmental and Biological Engineering, Nanjing University of Science and Technology Nanjing 210094 P. R. China sydeng@njust.edu.cn.

Xiyong ZhuoSchool of Environmental and Biological Engineering, Nanjing University of Science and Technology Nanjing 210094 P. R. China sydeng@njust.edu.cn.

Ying WuSchool of Environmental and Biological Engineering, Nanjing University of Science and Technology Nanjing 210094 P. R. China sydeng@njust.edu.cn.

Ying WanIntelligent Microsystem Technology and Engineering Center, School of Mechanical Engineering, Nanjing University of Science and Technology Nanjing 210094 P. R. China wanying@njust.edu.cn.ORCID https://orcid.org/0000-0002-3409-0492

Shengyuan DengSchool of Environmental and Biological Engineering, Nanjing University of Science and Technology Nanjing 210094 P. R. China sydeng@njust.edu.cn.ORCID https://orcid.org/0000-0002-6382-0976

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recent studies have shown that enzymes undergo chemotaxis up substrate gradients during catalysis. One important avenue to identify the molecular level origins of this phenomenon is the ligand-protein binding that occurs even in the absence of catalytic turnover. Here, the chemotaxis of zinc porphyrin as a cofactor mimic was observed by imposing a concentration gradient of organic amines in the microfluidic device. Their axial ligations led to the directed motions of porphyrin receptors. The dissociation constant for selected recognition could be obtained by measuring the chemotactic shift as a function of ligand content, which is associated with both the binding strength and the steric hindrance of the specific ligand. Finally, a statistical thermodynamic model was derived, relating the change of Gibbs free energy (Δ

Identifiers

PMID36540820

DOI10.1039/d2sc04849c

PMCPMC9728573

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LicenceCC BY-NC

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.