ArticlePlant communications2023
High temperature increases centromere-mediated genome elimination frequency and enhances haploid induction in Arabidopsis.
Article in Plant communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- The meiotic achilles' heel: vulnerability and resilience under environmental stress.Protoplasma · 2026Review
- Thermal Stress as a Tool for Manipulating Haploid Induction Rate in Brassica napus.Plant, cell & environment · 2026Article
- Diverse molecular mechanisms of in vivo haploid induction in plants: from fertilization interference to genome programming.Plant cell reports · 2026Review
- Targeted CENH3 protein depletion in egg cells enables highly efficient haploid induction.Plant communications · 2026Article
- The cenH3 assembly factor ZmKNL2 boosts haploid induction in maize.Plant biotechnology journal · 2025Article
- Exploring potential strategies for haploid induction based on double fertilization in plants.Plant biotechnology journal · 2025Review
- Efficient haploid induction in Arabidopsis thaliana by fine-tuning CENH3 function.Plant communications · 2025Article
- Ubiquitin-dependent proteolysis of KNL2 driven by APC/CCDC20 is critical for centromere integrity and mitotic fidelity.The Plant cell · 2025Article
- Article
- Centromere diversity and its evolutionary impacts on plant karyotypes and plant reproduction.The New phytologist · 2025Review
- Past innovations and future possibilities in plant chromosome engineering.Plant biotechnology journal · 2025Review
- Heat stress at the bicellular stage inhibits sperm cell development and transport into pollen tubes.Plant physiology · 2024Article
- Centromere sequence-independent but biased loading of subgenome-specific CENH3 variants in allopolyploid Arabidopsis suecica.Plant molecular biology · 2024Article
- Article
- The structure, function, and evolution of plant centromeres.Genome research · 2024Review
- Article
- Haploid induction: an overview of parental factor manipulation during seed formation.Frontiers in plant science · 2024Review
- Plant kinetochore complex: composition, function, and regulation.Frontiers in plant science · 2024Review
- Heat stress promotes haploid formation during CENH3-mediated genome elimination in Arabidopsis.Plant reproduction · 2023Article
- The impact of heat stress in plant reproduction.Frontiers in plant science · 2023Review
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Double haploid production is the most effective way to create true-breeding lines in a single generation. In Arabidopsis, haploid induction via mutation of the centromere-specific histone H3 (cenH3) has been shown when the mutant is outcrossed to the wild-type, and the wild-type genome remains in the haploid progeny. However, factors that affect haploid induction are still poorly understood. Here, we report that a mutant of the cenH3 assembly factor Kinetochore Null2 (KNL2) can be used as a haploid inducer when pollinated by the wild-type. We discovered that short-term temperature stress of the knl2 mutant increased the efficiency of haploid induction 10-fold. We also demonstrated that a point mutation in the CENPC-k motif of KNL2 is sufficient to generate haploid-inducing lines, suggesting that haploid-inducing lines in crops can be identified in a naturally occurring or chemically induced mutant population, avoiding the generic modification (GM) approach at any stage. Furthermore, a cenh3-4 mutant functioned as a haploid inducer in response to short-term heat stress, even though it did not induce haploids under standard conditions. Thus, we identified KNL2 as a new target gene for the generation of haploid-inducer lines and showed that exposure of centromeric protein mutants to high temperature strongly increases their haploid induction efficiency.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.