Evidence map›Paper›PMID 36540015›Full record

ArticleCell biochemistry and function2023

Increased PHOSPHO1 and alkaline phosphatase expression during the anabolic bone response to intermittent parathyroid hormone delivery.

Dean A Houston, Louise A Stephen, Soher N Jayash, Katherine Myers, Kirsty Little, Mark Hopkinson, Andrew A Pitsillides, Vicky E MacRae, Jose Luis Millan, Katherine A Staines and 1 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell biochemistry and function, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 2 countries.

Dean A HoustonFunctional Genetics Division, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK.
Louise A StephenFunctional Genetics Division, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK.ORCID http://orcid.org/0000-0001-6795-0383
Soher N JayashFunctional Genetics Division, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK.
Katherine MyersFunctional Genetics Division, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK.
Kirsty LittleFunctional Genetics Division, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK.
Mark HopkinsonComparative Biomedical Sciences, The Royal Veterinary College, London, UK.ORCID http://orcid.org/0000-0002-9508-8652
Andrew A PitsillidesComparative Biomedical Sciences, The Royal Veterinary College, London, UK.
Vicky E MacRaeFunctional Genetics Division, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK.
Jose Luis MillanHuman Genetics Program, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, USA.
Katherine A StainesSchool of Applied Sciences, Centre for Stress and Age-Related Disease, University of Brighton, Brighton, UK.
Colin FarquharsonFunctional Genetics Division, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK.ORCID http://orcid.org/0000-0002-4970-4039
Roslin Institute · GBRoyal Veterinary College · GBSanford Burnham Prebys Medical Discovery Institute · USUniversity of Brighton · GB

Funding

BB/J004316/1BB/J01446X/1Biotechnology and Biological Sciences Research Council
6 · The paper itself

Abstract

The administration of intermittent parathyroid hormone (iPTH) is anabolic to the skeleton. Recent studies with cultured osteoblasts have revealed that the expression of PHOSPHO1, a bone-specific phosphatase essential for the initiation of mineralisation, is regulated by PTH. Therefore, this study sought to determine whether the bone anabolic response to iPTH involves modulation of expression of Phospho1 and of other enzymes critical for bone matrix mineralisation. To mimic iPTH treatment, primary murine osteoblasts were challenged with 50 nM PTH for 6 h in every 48 h period for 8 days (4 cycles), 14 days (7 cycles) and 20 days (10 cycles) in total. The expression of both Phospho1 and Smpd3 was almost completely inhibited after 4 cycles, whereas 10 cycles were required to stimulate a similar response in Alpl expression. To explore the in vivo role of PHOSPHO1 in PTH-mediated osteogenesis, the effects of 14- and 28-day iPTH (80 µg/kg/day) administration was assessed in male wild-type (WT) and Phospho1

Indexed as

Alkaline PhosphataseParathyroid HormoneAnimalsBone and BonesBone DensityMaleMiceOsteoblastsOsteogenesisPhosphoric Monoester HydrolasesSphingomyelin PhosphodiesteraseAlkaline PhosphataseParathyroid HormonePHOSPHO1 protein, mousePhosphoric Monoester HydrolasesSmpd3 protein, mouseSphingomyelin Phosphodiesterasebone mineralisationintermittent PTHphosphatasePHOSPHO1SMPD3TNAP

Identifiers

PMID36540015
PMCPMC10946561
OpenAlexW4312065401

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.