Evidence map›Paper›PMID 36539820›Full record

ArticleBMC veterinary research2022

Guest edited collection serological study of SARS-CoV-2 antibodies in japanese cats using protein-A/G-based ELISA.

Ichiro Imanishi, Ryota Asahina, Shunji Hayashi, Jumpei Uchiyama, Masaharu Hisasue, Masahiro Yamasaki, Yoshiteru Murata, Shigeru Morikawa, Tetsuya Mizutani, Masahiro Sakaguchi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Low serological rate of SARS-CoV-2 in cats from military bases in Israel.Comparative immunology, microbiology and infectious diseases
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 8 institutions in 1 country.

Ichiro ImanishiDepartment of Microbiology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku Sagamihara-shi, Kanagawa, Kanagawa, Japan. ichiro.i@med.kitasato-u.ac.jp.ORCID http://orcid.org/0000-0002-4292-5533
Ryota AsahinaFaculty of Medicine, Department of Dermatology, Kyoto University, Kyoto, Japan.
Shunji HayashiDepartment of Microbiology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku Sagamihara-shi, Kanagawa, Kanagawa, Japan.
Jumpei UchiyamaDepartment of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
Masaharu HisasueCenter for Human and Animal Symbiosis Science, Azabu University, Kanagawa, Japan.
Masahiro YamasakiDepartment of Veterinary Internal Medicine, Iwate University, Iwate, Japan.
Yoshiteru MurataResearch and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Shigeru MorikawaFaculty of Veterinary Medicine, Department of Microbiology, Okayama University of Science, Ehime, Japan.
Tetsuya MizutaniResearch and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Masahiro SakaguchiInstitute of Tokyo Environmental Allergy, Tokyo, Japan.
Kitasato University · JPAzabu University · JPIwate University · JPKyoto University · JPMurata (Japan) · JPOkayama University · JPOkayama University of Science · JPTokyo University of Agriculture and Technology · JP

Funding

CSRD VA 1Japan Society for the Promotion of Science 21K14976Tokyo Metropolitan Government Grant funded the survey 2020-Univ-4
6 · The paper itself

Abstract

backgroundLittle is known about the epidemic status of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in cats in Japan due to insufficiently reliable seroepidemiological analysis methods that are easy to use in cats.

resultsWe developed a protein-A/G-based enzyme-linked immunosorbent assay (ELISA) to detect antibodies against SARS-CoV-2 in cats. The assay was standardized using positive rabbit antibodies against SARS-CoV-2. The ELISA results were consistent with those of a conventional anti-feline-immunoglobulin-G (IgG)-based ELISA. To test the protein-A/G-based ELISA, we collected blood samples from 1,969 cats that had been taken to veterinary clinics in Japan from June to July 2020 and determined the presence of anti-SARS-CoV-2 antibodies. Nine cats were found to have SARS-CoV-2 S1-specific IgG, of which 4 had recombinant receptor-binding domain-specific IgG. Of those 9 samples, one showed neutralizing activity. Based on these findings, we estimated that the prevalence of SARS-CoV-2 neutralizing antibodies in cats in Japan was 0.05% (1/1,969 samples). This prevalence was consistent with the prevalence of neutralizing antibodies against SARS-CoV-2 in humans in Japan according to research conducted at that time.

conclusionsProtein-A/G-based ELISA has the potential to be a standardized method for measuring anti-SARS-CoV-2 antibodies in cats. The infection status of SARS-CoV-2 in cats in Japan might be linked to that in humans.

Indexed as

Cat DiseasesCOVID-19AnimalsAntibodies, NeutralizingAntibodies, ViralCatsEnzyme-Linked Immunosorbent AssayImmunoglobulin GSARS-CoV-2Antibodies, NeutralizingAntibodies, ViralImmunoglobulin GCatsHealth risk behavioursSARS-CoV-2Seroepidemiological studies

Identifiers

PMID36539820
PMCPMC9767852
OpenAlexW4313311979

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.