Evidence map›Paper›PMID 36539609›Full record

ArticleAnalytical and bioanalytical chemistry2023

Rapid detection of Escherichia coli in dairy milk using static headspace-comprehensive two-dimensional gas chromatography.

Wan Sin Heng, Snehal R Jadhav, Maiken Ueland, Robert A Shellie

Abstract read
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In one paragraph

Article in Analytical and bioanalytical chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Wan Sin HengSchool of Exercise and Nutrition Sciences, CASS Food Research Centre, Deakin University, 221 Burwood Highway, Burwood, Australia.
Snehal R JadhavSchool of Exercise and Nutrition Sciences, CASS Food Research Centre, Deakin University, 221 Burwood Highway, Burwood, Australia.
Maiken UelandCentre for Forensic Science, School of Mathematical and Physical Sciences, University of Technology Sydney, 15 Broadway, Ultimo, Australia.
Robert A ShellieSchool of Exercise and Nutrition Sciences, CASS Food Research Centre, Deakin University, 221 Burwood Highway, Burwood, Australia. Robert.Shellie@utas.edu.au.
Deakin University · AUUniversity of Technology Sydney · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A new approach is introduced for rapid and reliable bacteria detection in food. Namely, static headspace-comprehensive two-dimensional gas chromatography (HS-GC × GC) with backflushing. The introduced approach provides fast detection of Escherichia coli (E. coli) in enriched ultra-high-temperature processed (UHT) dairy milk. The presence of E. coli may be indicated by detecting microbial volatile organic compounds emanating from test solutions inoculated with E. coli. In the present investigation, HS-GC × GC analysis is preceded by conventional enrichment in nutrient broth and inoculated samples are clearly discernable from controls following as little as 15 h sample enrichment. Headspace equilibration for 28 min followed by an 8 min GC × GC analysis of enriched test solutions reduces time-to-response by approximately one full day compared to conventional culture-based methods. The presence of ethanol, 1-propanol, and acetaldehyde may be used as a putative marker of E. coli contamination in milk and the introduced approach is able to detect single-cell initial bacterial load. Faster, reliable detection of pathogens and/or spoilage microbes in food products is desirable for the food industry. The described approach has great potential to complement the conventional workflow and be utilised for rapid microbial screening of foodstuff.

Indexed as

Escherichia coliVolatile Organic CompoundsAnimalsBacteriaEthanolGas Chromatography-Mass SpectrometryMilkEthanolVolatile Organic CompoundsBackflushEscherichia coliFood safetyGC × GCHeadspaceMetabolitesMicrobial volatile organic compoundsMVOC

Identifiers

PMID36539609
OpenAlexW4312067339

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.