Evidence map›Paper›PMID 36539418›Full record

ArticleCell death & disease2022

Astrocyte-derived exosomal nicotinamide phosphoribosyltransferase (Nampt) ameliorates ischemic stroke injury by targeting AMPK/mTOR signaling to induce autophagy.

Yang Deng, Rui Duan, Wangli Ding, Qiuchen Gu, Manman Liu, Junshan Zhou, Jianguo Sun, Junrong Zhu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell death & disease, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
5.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 74 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Yang Deng *Department of Pharmacy, Nanjing First Hospital, Nanjing Medical University, 210006, Nanjing, China.
Rui Duan *Department of Neurology, Nanjing First Hospital, Nanjing Medical University, 210006, Nanjing, China.
Wangli Ding *Department of Pharmacy, Nanjing First Hospital, Nanjing Medical University, 210006, Nanjing, China.
Qiuchen GuDepartment of Pharmacy, Nanjing First Hospital, Nanjing Medical University, 210006, Nanjing, China.
Manman LiuDepartment of Pharmacy, Nanjing First Hospital, Nanjing Medical University, 210006, Nanjing, China.
Junshan ZhouDepartment of Neurology, Nanjing First Hospital, Nanjing Medical University, 210006, Nanjing, China.
Jianguo SunKey Lab of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, 210009, Nanjing, China. jgsun@cpu.edu.cn.
Junrong ZhuDepartment of Pharmacy, Nanjing First Hospital, Nanjing Medical University, 210006, Nanjing, China. junrong_zhu@aliyun.com.ORCID 0000-0002-1647-7917
China Pharmaceutical University · CNNanjing Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Acute ischemic stroke (AIS) is a global cerebrovascular disease with high disability and mortality, which has no effective therapy. Studies have demonstrated that astrocyte-derived exosomes (ADEXs) provided neuroprotection in experimental stroke models. Nevertheless, the role of exosomes derived from oxygen-glucose-deprivation/reoxygenation-stimulated astrocytes (OGD/R-stimulated astrocytes; OGD/R-ADEXs) in AIS remains largely unknown. Here, we found that OGD/R-ADEXs significantly reduced OGD/R-induced neuronal death and promoted neuronal autophagy. These effects were reversed when astrocytes were pretreated with GW4869, an exosome secretion inhibitor, or when hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) was knocked down. Neuroprotection was also observed during treatment with OGD/R-ADEXs in vivo. Further studies showed that Nampt, played a vital effect in the regulation of autophagy, was significantly increased in OGD/R-ADEXs. Knockdown of Nampt in astrocytes abolished the above-mentioned effects of OGD/R-ADEXs. Mechanistically, Nampt increased autophagy and decreased cell death by modulating AMPK/mTOR signaling, which recognized as a key signaling pathway of autophagy after AIS. Collectively, these results showed that Nampt released by OGD/R-ADEXs ameliorated acute ischemic stroke during neuronal injury by targeting AMPK/mTOR signaling to induce autophagy. Our study revealed a new key factor in the secretion of exosomes by OGD/R astrocytes, which regulated autophagy and induced neuroprotection in a mouse stroke model.

Indexed as

Ischemic StrokeStrokeAMP-Activated Protein KinasesAnimalsAstrocytesAutophagyDisease Models, AnimalGlucoseMiceNicotinamide PhosphoribosyltransferaseOxygenSignal TransductionTOR Serine-Threonine KinasesAMP-Activated Protein KinasesGlucoseNicotinamide PhosphoribosyltransferaseOxygenTOR Serine-Threonine Kinases

Identifiers

PMID36539418
PMCPMC9767935
OpenAlexW4312113092

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.