Evidence map›Paper›PMID 36537621›Full record

ReviewCytometry. Part B, Clinical cytometry2023

Multiparameter flow cytometry in the evaluation of myelodysplasia: Analytical issues: Recommendations from the European LeukemiaNet/International Myelodysplastic Syndrome Flow Cytometry Working Group.

Anna Porwit, Marie C Béné, Carolien Duetz, Sergio Matarraz, Uta Oelschlaegel, Theresia M Westers, Orianne Wagner-Ballon, Shahram Kordasti, Peter Valent, Frank Preijers and 25 more

Open access · bronzeAbstract readReview
In one paragraph

Review in Cytometry. Part B, Clinical cytometry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed, 1 pooled it
6.9field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.

  1. Guideline
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  6. [Chinese guidelines for diagnosis and treatment of myelodysplastic neoplasms (2026)].Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

35 authors at 20 institutions in 11 countries.

Anna PorwitDivision of Oncology and Pathology, Department of Clinical Sciences, Faculty of Medicine, Lund University, Lund, Sweden.ORCID 0000-0003-0709-2713
Marie C BénéHematology Biology, Nantes University Hospital, CRCINA Inserm 1232, Nantes, France.ORCID 0000-0002-6569-7414
Carolien DuetzDepartment of Hematology, Amsterdam UMC, VU University Medical Center Cancer Center Amsterdam, Amsterdam, The Netherlands.
Sergio MatarrazCancer Research Center (IBMCC-USAL/CSIC), Department of Medicine and Cytometry Service, Institute for Biomedical Research of Salamanca (IBSAL) and CIBERONC, University of Salamanca, Salamanca, Spain.
Uta OelschlaegelDepartment of Internal Medicine, University Hospital Carl-Gustav-Carus, TU Dresden, Dresden, Germany.
Theresia M WestersDepartment of Hematology, Amsterdam UMC, VU University Medical Center Cancer Center Amsterdam, Amsterdam, The Netherlands.
Orianne Wagner-BallonDepartment of Hematology and Immunology, Assistance Publique-Hôpitaux de Paris, University Hospital Henri Mondor, Créteil, France.ORCID 0000-0001-9071-6683
Shahram KordastiComprehensive Cancer Centre, King's College London, London, UK.
Peter ValentDepartment of Internal Medicine I, Division of Hematology & Hemostaseology and Ludwig Boltzmann Institute for Hematology and Oncology, Medical University of Vienna, Vienna, Austria.
Frank PreijersLaboratory of Hematology, Department of Laboratory Medicine, Radboud University Medical Center, Nijmegen, The Netherlands.
Canan AlhanDepartment of Hematology, Amsterdam UMC, VU University Medical Center Cancer Center Amsterdam, Amsterdam, The Netherlands.
Frauke BellosMLL Munich Leukemia Laboratory, Munich, Germany.
Peter BettelheimDepartment of Hematology, Ordensklinikum Linz, Elisabethinen, Linz, Austria.
Kate BurburyDepartment of Haematology, Peter MacCallum Cancer Centre, & University of Melbourne, Melbourne, Australia.
Nicolas ChapuisLaboratory of Hematology, Assistance Publique-Hôpitaux de Paris, Centre-Université de Paris, Cochin Hospital, Paris, France.ORCID 0000-0003-0601-3948
Eline CremersDivision of Hematology, Department of Internal Medicine, Maastricht University Medical Center, Maastricht, The Netherlands.
Matteo G Della PortaIRCCS Humanitas Research Hospital, Milan, Italy.
Alan DunlopDepartment of Haemato-Oncology, Royal Marsden Hospital, London, UK.
Lisa Eidenschink-BrodersenHematoLogics, Inc., Seattle, Washington, USA.
Patricia FontDepartment of Hematology, Hospital General Universitario Gregorio Marañon-IiSGM, Madrid, Spain.
Michaela FontenayLaboratory of Hematology, Assistance Publique-Hôpitaux de Paris, Centre-Université de Paris, Cochin Hospital, Paris, France.
Willemijn HoboDepartment of Internal Medicine I, Division of Hematology & Hemostaseology and Ludwig Boltzmann Institute for Hematology and Oncology, Medical University of Vienna, Vienna, Austria.
Robin IrelandDepartment of Haematology and SE-HMDS, King's College Hospital NHS Foundation Trust, London, UK.
Ulrika JohanssonLaboratory Medicine, SI-HMDS, University Hospitals Bristol and Weston NHS Foundation Trust, Bristol, UK.
Michael R LokenHematoLogics, Inc., Seattle, Washington, USA.
Kiyoyuki OgataMetropolitan Research and Treatment Centre for Blood Disorders (MRTC Japan), Tokyo, Japan.
Alberto OrfaoCancer Research Center (IBMCC-USAL/CSIC), Department of Medicine and Cytometry Service, Institute for Biomedical Research of Salamanca (IBSAL) and CIBERONC, University of Salamanca, Salamanca, Spain.
Katherina PsarraDepartment of Immunology - Histocompatibility, Evangelismos Hospital, Athens, Greece.
Leonie SaftClinical Pathology and Cancer Diagnostics, Karolinska University Hospital and Institute Solna, Stockholm, Sweden.
Dolores SubiraDepartment of Hematology, Flow Cytometry Unit, Hospital Universitario de Guadalajara, Guadalajara, Spain.ORCID 0000-0002-9485-0529
Jeroen Te MarveldeLaboratory Medical Immunology, Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.
Denise A WellsHematoLogics, Inc., Seattle, Washington, USA.
Vincent H J van der VeldenLaboratory Medical Immunology, Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.ORCID 0000-0001-9457-3763
Wolfgang KernMLL Munich Leukemia Laboratory, Munich, Germany.
Arjan A van de LoosdrechtDepartment of Hematology, Amsterdam UMC, VU University Medical Center Cancer Center Amsterdam, Amsterdam, The Netherlands.
Cancer Center Amsterdam · NLChromoLogic (United States) · USCentre National de la Recherche Scientifique · FRErasmus MC · NLInserm · FRMedical University of Vienna · ATMunich Leukemia Laboratory (Germany) · DEUniversidad de Salamanca · ESUniversity Hospitals Bristol NHS Foundation Trust · GBCenter for Cancer and Blood Disorders · USEvangelismos Hospital · GRHospital General Universitario Gregorio Marañón · ESHospital Universitario de Guadalajara · ESHumanitas University · ITKarolinska University Hospital · SEKing's College London · GBKrankenhaus der Elisabethinen · ATLund University · SEMaastricht University Medical Centre · NLRadboud University Nijmegen · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Multiparameter flow cytometry (MFC) is one of the essential ancillary methods in bone marrow (BM) investigation of patients with cytopenia and suspected myelodysplastic syndrome (MDS). MFC can also be applied in the follow-up of MDS patients undergoing treatment. This document summarizes recommendations from the International/European Leukemia Net Working Group for Flow Cytometry in Myelodysplastic Syndromes (ELN iMDS Flow) on the analytical issues in MFC for the diagnostic work-up of MDS. Recommendations for the analysis of several BM cell subsets such as myeloid precursors, maturing granulocytic and monocytic components and erythropoiesis are given. A core set of 17 markers identified as independently related to a cytomorphologic diagnosis of myelodysplasia is suggested as mandatory for MFC evaluation of BM in a patient with cytopenia. A myeloid precursor cell (CD34

Indexed as

Myelodysplastic SyndromesAntigens, CD34Flow CytometryGranulocytesHumansImmunophenotypingMonocytesAntigens, CD34consensusELNflow cytometrymyelodysplastic syndromesstandardization

Identifiers

PMID36537621
PMCPMC10107708
OpenAlexW4312018385

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.