ArticleMicrobial cell factories2022
Green immobilization and efficient proliferation of Escherichia coli cells in polyvinyl alcohol hydrogel membranes by unidirectional nanopore dehydration.
Article in Microbial cell factories, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Granular Hydrogel Composites as Matrices for Engineered Living Materials.ACS applied polymer materials · 2026Article
- Bacterial growth in poly(vinyl alcohol) hydrogel with nutrients and indicator.Scientific reports · 2026Article
- Exploring interactions of Aliivibrio fischeri with water-soluble polymers using bioluminescence and Raman microspectroscopy.PloS one · 2025Article
- An Overview of Microorganisms Immobilized in a Gel Structure for the Production of Precursors, Antibiotics, and Valuable Products.Gels (Basel, Switzerland) · 2024Review
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2 authors.
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Abstract
backgroundThe immobilized technology for microbial or cells has the advantages of high microbial activity, high microbial density per unit space, good tolerance, strong shock, load resistance, high processing efficiency, and high reuse rate. It is now widely used in environmental remediation, water quality treatment, biodegradation, food industry, chemical analysis, energy development, medicine and pharmaceuticals, and other fields.
resultsA novel Escherichia coli cell-immobilizing polyvinyl alcohol hydrogel membrane (ECI-PVAHM) was prepared by unidirectional nanopore dehydration (UND) from a 10% polyvinyl alcohol (PVA) aqueous solution containing enhanced green fluorescent protein-labeled E. coli. This bacteria-loaded film has high water stability, flexibility, transparency, and mechanical robustness. Its tensile strength, elongation rate, and swelling rate are in the ranges 0.66-0.90 MPa, 300-390%, and 330-800%, respectively. The effective bacterial load of ECI-PVAHM is 2.375 × 10
conclusionThe above results show that the cell division, proliferation ability, and metabolic activity of immobilized E. coli were not affected by the physical barrier of the porous network structure of the hydrogel. This UND-based ECI-PVAHM has potential applications in molecular biology, biopharmaceutical expression and production, bioreactors, and fuel cells.
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