Evidence map›Paper›PMID 36535546›Full record

ArticleProtein expression and purification2023

Time- and cost-efficient bacterial expression and purification of potato apyrase.

Jordan A Karim, Nevin A Lambert, Augen A Pioszak

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein expression and purification, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Jordan A KarimDepartment of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA.
Nevin A LambertDepartment of Pharmacology and Toxicology, Medical College of Georgia, Augusta University, Augusta, GA, 30912, USA.
Augen A PioszakDepartment of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA. Electronic address: augen-pioszak@ouhsc.edu.
University of Oklahoma Health Sciences Center · USAugusta University · US

Funding

RAMP-altered Class B GPCR Hormone RecognitionR01GM104251 · NIGMS · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI PIOSZAK, AUGEN A · 2012 to 2024
$4.2M
Conventional and unconventional GPCR-G protein couplingR35GM145284 · NIGMS · AUGUSTA UNIVERSITY · PI Nevin Alan Lambert · 2022 to 2026
$1.8M
Direct assessment of GPCR-transducer coupling and G protein subtype biasR01GM130142 · NIGMS · AUGUSTA UNIVERSITY · PI LAMBERT, NEVIN ALAN · 2018 to 2021
$1.5M
NIGMS NIH HHS R01 GM104251NIGMS NIH HHS R01 GM130142NIGMS NIH HHS R35 GM145284
6 · The paper itself

Abstract

Apyrase from potato (Solanum tuberosum) is a divalent metal ion-dependent enzyme that catalyzes the hydrolysis of nucleoside di- and tri-phosphates with broad substrate specificity. The enzyme is widely used to manipulate nucleotide levels such as in the G protein-coupled receptor (GPCR) field where it is used to deplete guanine nucleotides to stabilize nucleotide-free ternary agonist-GPCR-G protein complexes. Potato apyrase is available commercially as the native enzyme purified from potatoes or as a recombinant protein, but these are prohibitively expensive for some research applications. Here, we report a relatively simple method for the bacterial production of soluble, active potato apyrase. Apyrase has several disulfide bonds, so we co-expressed the enzyme bearing a C-terminal (His)

Indexed as

ApyraseSolanum tuberosumEscherichia coliGTP-Binding ProteinsReceptors, G-Protein-CoupledRecombinant ProteinsApyraseGTP-Binding ProteinsReceptors, G-Protein-CoupledRecombinant ProteinsCibacron blue dye affinity chromatographyE. coli trxB gorGPCR-G protein coupling BRET assayNucleotide phosphohydrolaseRecombinant protein expression

Identifiers

PMID36535546
PMCPMC9807108
OpenAlexW4311632512

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.