Evidence map›Paper›PMID 36533601›Full record

ArticleSarcoidosis, vasculitis, and diffuse lung diseases : official journal of WASOG2022

Metagenomic sequencing of the bronchoalveolar lavage extracellular virome and cellular transcriptome of sarcoidosis patients does not detect rubella virus.

Emma L Keeler, Milica Vukmirovic, Xiting Yan, Kristin Gulino, Elodie Ghedin, Naftali Kaminski, Kathleen E Sullivan, Frederic D Bushman, Ronald G Collman, Misha Rosenbach

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Sarcoidosis, vasculitis, and diffuse lung diseases : official journal of WASOG, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 86% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 1 country.

Emma L KeelerDepartment of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA. emkeeler@sas.upenn.edu.
Milica VukmirovicDepartment of Medicine, Yale University School of Medicine, New Haven, CT, USA. milica.vukmirovic@utoronto.ca.
Xiting YanDepartment of Medicine, Yale University School of Medicine, New Haven, CT, USA. xiting.yan@yale.edu.
Kristin GulinoDepartment of Biology, Center for Genomics and Systems Biology, New York University, New York, NY, USA. kristen.gulino@nyu.edu.
Elodie GhedinDepartment of Biology, Center for Genomics and Systems Biology, New York University, New York, NY, USA and Laboratory of Parasitic Diseases, NIAID, NIH, Washington, DC, USA. elodie.ghedin@nih.gov.
Naftali KaminskiDepartment of Medicine, Yale University School of Medicine, New Haven, CT, USA. naftali.kaminski@yale.edu.
Kathleen E SullivanDepartment of Pediatrics, University of Pennsylvania School of Medicine, and Children's Hospital of Philadelphia, Philadelphia, PA, USA. sullivank@email.chop.edu.
Frederic D BushmanDepartment of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA. bushman@pennmedicine.upenn.edu.
Ronald G CollmanDepartments of Medicine and Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA. collmanr@pennmedicine.upenn.edu.
Misha RosenbachDepartment of Dermatology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA. Misha.Rosenbach@pennmedicine.upenn.edu.
University of Pennsylvania · USYale University · USChildren's Hospital of Philadelphia · USID Genomics (United States) · USNew York University · US

Funding

Yale Clinical and Translational Science Award (U Component)UL1TR001863 · NCATS · YALE UNIVERSITY · PI John H. Krystal, LUCILA OHNO-MACHADO · 2016 to 2026
$102.9M
Graph Learning of Cell-cell Communications in Spatial TranscriptomicsR01LM014087 · NLM · YALE UNIVERSITY · PI WANG, ZUOHENG, YAN, XITING · 2022 to 2025
$1.5M
NCATS NIH HHS UL1 TR001863NLM NIH HHS R01 LM014087
6 · The paper itself

Abstract

backgroundSarcoidosis is a multisystem granulomatous inflammatory disease of unclear etiology that involves the lung, skin and other organs, with an unknown antigenic trigger. Recently, evidence has been found in both immune deficient and immune competent patients for rubella virus in cutaneous granulomas. These granulomatous lesions share overlapping features with cutaneous sarcoidosis, raising the question of rubella virus in sarcoidosis.

objectiveTo investigate the presence of rubella virus in sarcoidosis lung samples.

methodsWe employed metagenomic sequencing to interrogate extracellular virome preparations and cellular transcriptomes from bronchoalveolar lavage (BAL) of 209 sarcoidosis patients for rubella virus sequences.

resultsWe found no evidence for rubella virus genomes in acellular fluid or rubella virus gene expression in BAL cells of sarcoidosis patients.

conclusionsThese findings argue against rubella virus infection or persistence within the lung at time of sampling as a sarcoidosis trigger.

Identifiers

PMID36533601
PMCPMC9798337
OpenAlexW4311927721

What OpenQuestion holds

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LicenceCC BY-NC-SA
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.