Evidence map›Paper›PMID 36533293›Full record

ArticleData in brief2022

RNASeq data from Indonesian recalcitrant and non-recalcitrant rice varieties on anther culture.

Siti Fatimah, Mohammad Syafii, Siti Zulaeha, Mega Dewi Haristianita, Devit Purwoko

Open access · goldAbstract read
In one paragraph

Article in Data in brief, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Siti FatimahAgrotechnology Study Program, Faculty of Agriculture, Trunojoyo University, Unijoyo Campus, Telang, PO. BOX 2 Kamal - Bangkalan, East Java, Indonesia.
Mohammad SyafiiAgrotechnology Study Program, Faculty of Agriculture, Trunojoyo University, Unijoyo Campus, Telang, PO. BOX 2 Kamal - Bangkalan, East Java, Indonesia.
Siti ZulaehaResearch Center for Genetic Engineering, Research Organization for Life Sciences and Environment, National Research and Innovation Agency, Building No. 630 PUSPIPTEK South Tangerang Banten 15314, Indonesia.
Mega Dewi HaristianitaThe University of Tokyo 7 Chome-3-1 Hongo, Bunkyo City, Tokyo 113-8654, Jepang.
Devit PurwokoResearch Center for Genetic Engineering, Research Organization for Life Sciences and Environment, National Research and Innovation Agency, Building No. 630 PUSPIPTEK South Tangerang Banten 15314, Indonesia.
National Research and Innovation Agency · IDTrunojoyo University · IDThe University of Tokyo · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The assembly of dihaploid rice plants through anther culture was constrained due to the recalcitrant properties. A comprehensive investigation of gene expression patterns among rice varieties with recalcitrant and non-recalcitrant anthers will help to understand the cellular mechanisms and biological processes of recalcitrant properties in rice anther cultures. Therefore, we performed RNA sequencing and analysis on the anthers of three selected Indonesian rice varieties with opposite recalcitrant properties. The varieties are Fatmawati with non-recalcitrant properties, IR64 recalcitrant and Tarabas unknown. The Illumina NextSeq PE150 sequencer was used to generate a total crude nucleotide of approximately 41.21 Gb in size. From 272,239,682 total paired final raw reads, 137,343,391 total net reads were obtained and uploaded to NCBI's Sequence Read Archive (SRA) repository under BioProject accession number PRJNA856048. This dataset allowed us to identify and profile all expressed genes with functions associated with recalcitrant and non-recalcitrant properties. In addition, the transcriptome data obtained will be valuable for the discovery of potential gene markers and functional SNPs associated with functional traits to assist rice breeding programs through the development of Marker Assisted Selection (MAS).

Indexed as

Anther cultureDihaploidOryza sativaRecalcitrantTranscriptomics

Identifiers

PMID36533293
PMCPMC9747646
OpenAlexW4309203692

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.