Evidence map›Paper›PMID 36532696›Full record

ArticleJournal of mass spectrometry and advances in the clinical lab2022

LC-MS/MS method for simultaneous quantification of ten antibiotics in human plasma for routine therapeutic drug monitoring.

Mirjana Radovanovic, Richard O Day, Graham D R Jones, Peter Galettis, Ross L G Norris

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of mass spectrometry and advances in the clinical lab, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 22 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 1 country.

Mirjana RadovanovicSt Vincent's Clinical School, University of New South Wales, Darlinghurst, NSW, Australia.
Richard O DaySt Vincent's Clinical School, University of New South Wales, Darlinghurst, NSW, Australia.
Graham D R JonesSt Vincent's Clinical School, University of New South Wales, Darlinghurst, NSW, Australia.
Peter GalettisCentre for Drug Repurposing and Medicines Research, University of Newcastle, Callaghan, NSW, Australia.
Ross L G NorrisSt Vincent's Clinical School, University of New South Wales, Darlinghurst, NSW, Australia.
St Vincent's Clinic · AUSt Vincent's Hospital Sydney · AUUniversity of Newcastle Australia · AUUNSW Sydney · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Optimizing antimicrobial therapy to attain drug exposure that limits the emergence of resistance, effectively treats the infection, and reduces the risk of side effects is of a particular importance in critically ill patients, in whom normal functions are augmented or/and are infected with pathogens less sensitive to treatment. Achievement of these goals can be enhanced by therapeutic drug monitoring (TDM) for many antibiotics. A liquid chromatography tandem mass spectrometry (LC-MS/MS) method is presented here for simultaneous quantification of ten antimicrobials: cefazolin (CZO), cefepime (CEP), cefotaxime (CTA), ceftazidime (CTZ), ciprofloxacin (CIP), flucloxacillin (FLU), linezolid (LIN), meropenem (MER), piperacillin (PIP) and tazobactam (TAZ) in human plasma. Methods: Plasma samples were precipitated with acetonitrile and injected into the LC-MS/MS. Chromatographic separation was on a Waters Acquity BEH C Results: The calibration curves were linear across the tested concentration ranges (0.5-250, CZO, CEP, CTA, CTZ and FLU; 0.2-100, MER and TAZ; 0.1-50, CIP and LIN and 1-500 mg/L, PIP). The intra and inter-day imprecision was < 11 %. Accuracy ranged from 95 to 114 %. CTZ and MER showed ionization suppression while CIP showed ionization enhancement, which was normalized with the use of the internal standard. Conclusion: An LC-MS/MS method for simultaneous quantification of ten antimicrobials in human plasma was developed for routine TDM.

Indexed as

AntimicrobialsCEP, cefepimeCIP, ciprofloxacinCTA, cefotaximeCTZ, ceftazidimeCV, coefficient of variationCZO, cefazolinESI, electrospray ionizationFLU, flucloxacillinHPLC, high performance liquid chromatographyICU, intensive care unitLC–MS/MSLC–MS/MS, liquid chromatography tandem mass spectrometryLIN, linezolidLLOQ, lower limit of quantificationMER, meropenemMIC, minimum inhibitory concentrationMRM, multiple reaction monitoringNOR, norfloxacinPIP, piperacillinPK, pharmacokineticQC, quality controlr2, coefficient of determinationRoutine therapeutic drug monitoringR, resistant organismRt, retention timeRT, room temperatureSIL-IS, stable isotope labelled internal standardS, susceptible, wild type organismTAZ, tazobactamTDM, therapeutic drug monitoringULOQ, upper limit of quantitationβ-Lactams

Identifiers

PMID36532696
PMCPMC9756784
OpenAlexW4309277497

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.