Evidence map›Paper›PMID 36532688›Full record

ArticleBio-protocol2022

Fluorescence Screens for Identifying Central Nervous System-Acting Drug-Biosensor Pairs for Subcellular and Supracellular Pharmacokinetics.

Zoe G Beatty, Anand K Muthusamy, Elizabeth K Unger, Dennis A Dougherty, Lin Tian, Loren L Looger, Amol V Shivange, Kallol Bera, Henry A Lester, Aaron L Nichols

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Selective Serotonin Reuptake Inhibitors within Cells: Temporal Resolution in Cytoplasm, Endoplasmic Reticulum, and Membrane.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Zoe G BeattyDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, USA.
Anand K MuthusamyDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, USA.
Elizabeth K UngerDepartment of Biochemistry and Molecular Medicine and the Center for Neuroscience, University of California at Davis, Davis, USA.
Dennis A DoughertyDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, USA.
Lin TianDepartment of Biochemistry and Molecular Medicine and the Center for Neuroscience, University of California at Davis, Davis, USA.
Loren L LoogerHHMI Janelia Research Campus, Ashburn, USA.
Amol V ShivangeDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, USA.
Kallol BeraDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, USA.
Henry A LesterDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, USA.
Aaron L NicholsDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, USA.
California Institute of Technology · USJanelia Research Campus · US

Funding

PREDOCTORAL TRAINING IN BIOLOGY AND CHEMISTRYT32GM007616 · NIGMS · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI STERNBERG, PAUL WARREN · 1985 to 2019
$22.9M
Fluorescent biosensors for subcellular pharmacokineticsR01GM123582 · NIGMS · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI LESTER, HENRY A. · 2016 to 2021
$3.8M
Predoctoral Training in Quantitative NeuroscienceT32NS105595 · NINDS · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI MARKUS MEISTER · 2019 to 2026
$2.6M
Nicotine Inside NeuronsR21DA043829 · NIDA · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI LESTER, HENRY A. · 2017 to 2018
$454k
Opioids inside OrganellesR21DA049140 · NIDA · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI LESTER, HENRY A. · 2019 to 2020
$452k
Ketamine-Class Antidepressants in VesiclesR21MH120823 · NIMH · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI LESTER, HENRY A. · 2019 to 2020
$452k
NIDA NIH HHS R21 DA043829NIDA NIH HHS R21 DA049140NIGMS NIH HHS R01 GM123582NIGMS NIH HHS T32 GM007616NIMH NIH HHS R21 MH120823NINDS NIH HHS T32 NS105595
6 · The paper itself

Abstract

Subcellular pharmacokinetic measurements have informed the study of central nervous system (CNS)-acting drug mechanisms. Recent investigations have been enhanced by the use of genetically encoded fluorescent biosensors for drugs of interest at the plasma membrane and in organelles. We describe screening and validation protocols for identifying hit pairs comprising a drug and biosensor, with each screen including 13-18 candidate biosensors and 44-84 candidate drugs. After a favorable hit pair is identified and validated via these protocols, the biosensor is then optimized, as described in other papers, for sensitivity and selectivity to the drug. We also show sample hit pair data that may lead to future intensity-based drug-sensing fluorescent reporters (iDrugSnFRs). These protocols will assist scientists to use fluorescence responses as criteria in identifying favorable fluorescent biosensor variants for CNS-acting drugs that presently have no corresponding biosensor partner.

Indexed as

BiosensorCNS-acting drugsDirected evolutionFluorescence screeningiDrugSnFRPharmacokinetics

Identifiers

PMID36532688
PMCPMC9724018
OpenAlexW4312311491

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.