ArticleFrontiers in microbiology2022
Rapid visual detection of anisakid nematodes using recombinase polymerase amplification and SYBR Green I.
Article in Frontiers in microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- A miniaturized, frugal RPA assay for genus-level detection of Paracoccidioides spp. in resource-limited endemic settings.PLoS neglected tropical diseases · 2026Article
- Rapid and Visual Detection of Muscovy Duck Reovirus Using a Reverse Transcription Recombinase-Aided Amplification Assay in a Lyophilized Format for Point-of-Care Applications.Transboundary and emerging diseases · 2026Article
- Rapid visual detection of bovine viral diarrhea virus (BVDV) using recombinase polymerase amplification with SYBR green I.BMC veterinary research · 2025Article
- Comparative study of Taqman-based qPCR assay for the detection of Anisakis simplex and Pseudoterranova decipiens.PloS one · 2025Article
- Rapid and Ultrasensitive Detection ofMolecules (Basel, Switzerland) · 2024Article
- A recombinase polymerase amplification-SYBR Green I assay for the rapid and visual detection of Brucella.Folia microbiologica · 2024Article
- Recombinase polymerase amplification combined with lateral flow dipstick assay for rapid visual detection ofHeliyon · 2024Article
- Re-evaluation of certain aspects of the EFSA Scientific Opinion of April 2010 on risk assessment of parasites in fishery products, based on new scientific data. Part 1: ToRs1-3.EFSA journal. European Food Safety Authority · 2024Article
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Authors and funding
10 authors.
Funding
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Abstract
Anisakidosis is a food-borne parasitic disease (FBPD) caused by the third-stage larvae of the family Anisakidae. Therefore, it is important to develop a simple, rapid and equipment-free detection method for anisakids in fish samples or seafood since current methods are time-consuming and require complex instruments. In this study, a recombinase polymerase amplification (RPA)-based method was established for the first time to detect anisakids by targeting the internal transcribed spacer (ITS) regions. The detection results were visualized by including SYBR Green I (SG) in the method. The sensitivity of RPA-SG assay was 10
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