Evidence map›Paper›PMID 36531118›Full record

ArticleInternational journal of nanomedicine2022

Small Extracellular Vesicles Released from Bioglass/Hydrogel Scaffold Promote Vascularized Bone Regeneration by Transferring miR-23a-3p.

Hongxing Hu, Hang Zhang, Ziheng Bu, Zhongtang Liu, Fang Lv, Mingmang Pan, Xuan Huang, Liming Cheng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of nanomedicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
43citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

43 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Extracellular Vesicles and Their Role in Osteogenesis.Bioengineering (Basel, Switzerland) · 2026
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  10. Study onMaterials today. Bio · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Hongxing HuDepartment of Orthopedics Tongji Hospital Affiliated to Tongji University, Tongji University School of Medicine, Shanghai, People's Republic of China.
Hang ZhangSchool of Mechanical Engineering, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Ziheng BuDepartment of Orthopedics Changhai Hospital Affiliated to the Second Military Medical University, Shanghai, People's Republic of China.
Zhongtang LiuDepartment of Orthopedics Changhai Hospital Affiliated to the Second Military Medical University, Shanghai, People's Republic of China.
Fang LvShanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Science and School of Life Science, East China Normal University, Shanghai, People's Republic of China.
Mingmang PanDepartment of orthopedics, Shanghai Fengxian District Central Hospital, Shanghai, People's Republic of China.
Xuan HuangDepartment of Orthopedics Changhai Hospital Affiliated to the Second Military Medical University, Shanghai, People's Republic of China.
Liming ChengDepartment of Orthopedics Tongji Hospital Affiliated to Tongji University, Tongji University School of Medicine, Shanghai, People's Republic of China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: The treatment of critical-size bone defect is a great difficulty in orthopedics. Osteogenesis and angiogenesis are critical issue during the process of bone repair and remodeling. Mesenchymal stem cells (MSCs)-derived exosomes have the same therapeutic effect to MSCs-based therapies. The effect of human umbilical cord MSCs-derived sEVs (hUC-MSCs-sEVs) on vascularized bone regeneration and the potential mechanism remains to be investigated. Herein, we aimed to explore the therapeutic effect and the mechanism of hUC-MSCs-sEVs on critical-size bone defect. Methods: To investigate the potential osteogenesis and angiogenesis effects of sEVs in vitro, we extracted sEVs from hUC-MSCs, and then sEVs were co-incubated with BMSCs and HUVECs. We next investigated the effect and potential mechanism of sEVs on the effects of osteogenesis and angiogenesis. We fabricated 3D-printed bioglass scaffold with Gelma/nanoclay hydrogel coatings to load sEVs (BG-gel-sEVs) to ensure in vivo sustained efficacy of sEVs. Finally, the skull defect model was used to evaluate the capacity of vascularized bone regeneration of the composited scaffolds. Results: hUC-MSCs-sEVs facilitated calcium deposition and the endothelial network formation, inducing osteogenic differentiation and angiogenesis by delivering miR-23a-3p to activate PTEN/AKT signaling pathway. Additionally, the BG-gel-sEVs composited scaffold achieved vascularized bone regeneration in vivo. Conclusion: This finding illuminated that hUC-MSCs-sEVs promoted osteogenesis and angiogenesis by delivering miR-23a-3p to activate PTEN/AKT signaling pathway, achieving vascularized bone regeneration.

Indexed as

ExosomesMicroRNAsBone RegenerationCeramicsHumansHydrogelsOsteogenesisProto-Oncogene Proteins c-aktTissue ScaffoldsBioglassHydrogelsMicroRNAsProto-Oncogene Proteins c-aktangiogenesisbioglass scaffoldsbone regenerationmiR-23a-3psmall extracellular vesicles

Identifiers

PMID36531118
PMCPMC9749034

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.