ArticleThe Journal of reproduction and development2023
Removal of sperm tail using trypsin and pre-activation of oocyte facilitates intracytoplasmic sperm injection in mice and rats.
Article in The Journal of reproduction and development, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 7 citations in OpenAlex.
- Highly efficient and low-mosaicismiScience · 2026Article
- Resurrection of chromosomes from frozen animals by single chromosome transfer into mouse oocytes.Scientific reports · 2026Article
- Chloroquine inhibits artificial oocyte activation induced by ethanol or SrThe Journal of reproduction and development · 2025Article
- Extracting and analyzing micronuclei from mouse two-cell embryos fertilized with freeze-dried spermatozoa.Communications biology · 2025Article
- Method for long-term room temperature storage of mouse freeze-dried sperm.Scientific reports · 2025Article
- Lower developmental potential of rat zygotes produced by ooplasmic injection of testicular spermatozoa versus cauda epididymal spermatozoa.The Journal of reproduction and development · 2024Article
- The Uterine Melatonergic Systems of AANAT and Melatonin Membrane Receptor 2 (MT2) Are Essential for Endometrial Receptivity and Early Implantation in Mice.International journal of molecular sciences · 2023Article
Corrections and comments
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Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We examined various methods to enhance the accessibility of intracytoplasmic sperm injection (ICSI) technology to more users by making the technique easier, more efficient, and practical. First, the methods for artificially removing the mouse sperm tail were evaluated. Trypsin treatment was found to efficiently remove the sperm tails. The resultant sperm cells had a lower oocyte activation capacity; however, the use of activated oocytes resulted in the same fecundity as that of fresh, untreated sperm. Pre-activated oocytes were more resistant to physical damage, showed higher survival rates, and required less time per injection. Testing this method in rats yielded similar results, although the oocyte activation method was different. Remarkably, this method resulted in higher birth rates of rat progeny than with conventional methods of rat ICSI. Our method thereby streamlines mouse and rat ICSI, making it more accessible to laboratories across many disciplines.
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Registered trials
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