ArticleScientific reports2022
Draft genome sequencing and secretome profiling of Sclerotinia sclerotiorum revealed effector repertoire diversity and allied broad-host range necrotrophy.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 21 citations in OpenAlex.
- Copper Radical Oxidases Contribute to Virulence inPathogens (Basel, Switzerland) · 2026Article
- Comprehensive whole genome hybrid assembly unravels genes and secretome ofFrontiers in fungal biology · 2026Article
- Genomic Loci for Sclerotinia Stem Rot Resistance and Chlorophyll Stability inPlant-environment interactions (Hoboken, N.J.) · 2025Article
- Effects of Interphase and Interpulse Delays on Tissue Impedance and Pulsed Field Ablation.Annals of biomedical engineering · 2025Article
- Development and Characterization of New SSR Markers inPathogens (Basel, Switzerland) · 2025Article
- Trans-Kingdom sRNA Silencing inPathogens (Basel, Switzerland) · 2025Review
- Unveiling the molecular arsenal: genome sequencing andFrontiers in plant science · 2025Article
- An effector SsCVNH promotes the virulence of Sclerotinia sclerotiorum through targeting class III peroxidase AtPRX71.Molecular plant pathology · 2024Article
- A Glycosyl Hydrolase 5 Family Protein Is Essential for Virulence of Necrotrophic Fungi and Can Suppress Plant Immunity.International journal of molecular sciences · 2024Article
- Guest edited collection: fungal evolution and diversity.Scientific reports · 2023Article
- Utilization of crop wild relatives for biotic and abiotic stress management in Indian mustard [Frontiers in plant science · 2023Review
- Fungal effectors versus defense-related genes ofFrontiers in plant science · 2023Review
- Population and genome-wide association studies ofFrontiers in microbiology · 2023Article
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10 authors at 4 institutions in 1 country.
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Abstract
White mold commonly known as Sclerotinia sclerotiorum causes stem rot disease and has emerged as one of the major fungal pathogens of oilseed Brassica across the world. In the present study, consistently virulent S. sclerotiorum isolate "ESR-01" was sequenced and an assembly size of ~ 41 Mb with 328 scaffolds having N50 of 447,128 was obtained. Additionally, 27,450 single nucleotide polymorphisms (SNPs) were identified from 155 scaffolds against S. sclerotiorum 1980 isolate, with an average SNP density of ~ 1.5 per kb genome. 667 repetitive elements were identified and approximately comprised 7% of the total annotated genes. The DDE_1 with 454 in numbers was found to be the most abundant and accounts for 68% of the total predicted repetitive elements. In total, 3844 simple sequence repeats are identified in the 328 scaffolds. A total of 9469 protein-coding genes were predicted from the whole genome assembly with an average gene length of 1587 bp and their distribution as 230.95 genes per Mb in the genome. Out of 9469 predicted protein-coding genes, 529 genes were observed encoding the CAZymes (Carbohydrate-Active enzymes) capable of degradation of the complex polysaccharides. Glycosyltransferase (GT) families were most abundant (49.71%) among the predicted CAZymes and GT2 (23%), GT4 (20%), and glycoside hydrolase (GH) 23% with GH18 (11%) were the prominent cell wall degrading enzyme families in the ESR-01 secretome. Besides this, 156 genes essential for the pathogen-host interactions were also identified. The effector analysis in the whole genome proteomics dataset revealed a total of 57 effector candidates (ECs) and 27 of them were having their analogs whereas the remaining 30 were novel ones. Eleven selected ECs were validated experimentally by analyzing the expression profile of the ESR-01 isolate of S. sclerotiorum. Together, the present investigation offers a better understanding of the S. sclerotiorum genome, secretome, and its effector repertoire which will help in refining the present knowledge on S. sclerotiorum-Brassica interactions and necrotrophic lifestyle of the phytopathogen in general.
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