ArticleMolecular plant pathology2023
Exogenous double-stranded RNA inhibits the infection physiology of rust fungi to reduce symptoms in planta.
Article in Molecular plant pathology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 47 citations in OpenAlex.
- Genomic and Effector-Based Insights Into Austropuccinia psidii-Host Interactions Informing RNAi and Resistance Development.Molecular plant pathology · 2026Review
- Screening Various Bacterial-Produced Double-Stranded RNAs for Managing Asian Soybean Rust Disease Caused byPlants (Basel, Switzerland) · 2026Article
- Soil-free bioassays for testing novel control agents againstFrontiers in plant science · 2026Article
- Identifying potentially suitable and accessible refugia to mitigate impacts of an emerging disease on a rare tree.Conservation biology : the journal of the Society for Conservation Biology · 2025Article
- Learning from fungicide resistance: Evolutionary insights to guide RNAi-based control of fungal crop pathogens.Fungal biology reviews · 2025Article
- dsRNAmax: a multi-target chimeric dsRNA designer for safe and effective crop protection.NAR genomics and bioinformatics · 2025Article
- Genomics Research on the Road of Studying Biology and Virulence of Cereal Rust Fungi.Molecular plant pathology · 2025Review
- Review
- Genomics in plant pathogen identification and control.Frontiers in plant science · 2025Review
- The role of polymers in enabling RNAi-based technology for sustainable pest management.Nature communications · 2024Review
- Challenges and Opportunities Arising from Host-International journal of molecular sciences · 2024Review
- Exogenous Application of dsRNA in Plant Protection: Efficiency, Safety Concerns and Risk Assessment.International journal of molecular sciences · 2024Review
- Double-stranded RNA prevents and cures infection by rust fungi.Communications biology · 2023Article
- Improving RNA-based crop protection through nanotechnology and insights from cross-kingdom RNA trafficking.Current opinion in plant biology · 2023Review
- Mining theJournal of fungi (Basel, Switzerland) · 2023Article
- RNA-Based Control of Fungal Pathogens in Plants.International journal of molecular sciences · 2023Review
- RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi.International journal of molecular sciences · 2023Review
- Exogenous double-stranded RNA inhibits the infection physiology of rust fungi to reduce symptoms in planta.Molecular plant pathology · 2023Article
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Authors and funding
13 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rust fungi (Pucciniales) are a diverse group of plant pathogens in natural and agricultural systems. They pose ongoing threats to the diversity of native flora and cause annual crop yield losses. Agricultural rusts are predominantly managed with fungicides and breeding for resistance, but new control strategies are needed on non-agricultural plants and in fragile ecosystems. RNA interference (RNAi) induced by exogenous double-stranded RNA (dsRNA) has promise as a sustainable approach for managing plant-pathogenic fungi, including rust fungi. We investigated the mechanisms and impact of exogenous dsRNA on rust fungi through in vitro and whole-plant assays using two species as models, Austropuccinia psidii (the cause of myrtle rust) and Coleosporium plumeriae (the cause of frangipani rust). In vitro, dsRNA either associates externally or is internalized by urediniospores during the early stages of germination. The impact of dsRNA on rust infection architecture was examined on artificial leaf surfaces. dsRNA targeting predicted essential genes significantly reduced germination and inhibited development of infection structures, namely appressoria and penetration pegs. Exogenous dsRNA sprayed onto 1-year-old trees significantly reduced myrtle rust symptoms. Furthermore, we used comparative genomics to assess the wide-scale amenability of dsRNA to control rust fungi. We sequenced genomes of six species of rust fungi, including three new families (Araucariomyceaceae, Phragmidiaceae, and Skierkaceae) and identified key genes of the RNAi pathway across 15 species in eight families of Pucciniales. Together, these findings indicate that dsRNA targeting essential genes has potential for broad-use management of rust fungi across natural and agricultural systems.
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