Evidence map›Paper›PMID 36527930›Full record

ArticleVirology2023

SNAP23 is essential for germination of EV-D68 replication organelles.

Katelyn Miller, Michael A Wagner, Alagie Jassey, William T Jackson

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in Virology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 56% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Katelyn MillerDepartment of Microbiology and Immunology, University of Maryland School of Medicine, 685 W Baltimore St, Baltimore, MD, 21201, USA.
Michael A WagnerDepartment of Microbiology and Immunology, University of Maryland School of Medicine, 685 W Baltimore St, Baltimore, MD, 21201, USA.
Alagie JasseyDepartment of Microbiology and Immunology, University of Maryland School of Medicine, 685 W Baltimore St, Baltimore, MD, 21201, USA.
William T JacksonDepartment of Microbiology and Immunology, University of Maryland School of Medicine, 685 W Baltimore St, Baltimore, MD, 21201, USA. Electronic address: wjackson@som.umaryland.edu.
University of Maryland, Baltimore · US

Funding

Signaling Pathways in Innate ImmunityT32AI095190 · NIAID · UNIVERSITY OF MARYLAND BALTIMORE · PI Stefanie N. Vogel · 2012 to 2026
$4.7M
The roles of acidic autophagosomes in production of infectious poliovirusR01AI104928 · NIAID · UNIVERSITY OF MARYLAND BALTIMORE · PI JACKSON, WILLIAM T · 2014 to 2023
$3.8M
Enterovirus manipulation of autophagic trafficking pathwaysR01AI141359 · NIAID · UNIVERSITY OF MARYLAND BALTIMORE · PI JACKSON, WILLIAM T · 2019 to 2022
$1.5M
Cryo Upgrade of UMB EM Core FacilityS10RR026870 · NCRR · UNIVERSITY OF MARYLAND BALTIMORE · PI HSIA, RU-CHING · 2010 to 2010
$421k
NCRR NIH HHS S10 RR026870NIAID NIH HHS R01 AI104928NIAID NIH HHS R01 AI141359NIAID NIH HHS T32 AI095190
6 · The paper itself

Abstract

Picornaviruses rearrange host cell membranes to facilitate their own replication. Here we investigate the Qbc SNARE, SNAP23, which is found at the plasma membrane and plays roles in exocytosis. We found that knockdown of SNAP23 expression inhibits virus replication but not release from cells. Knocking down SNAP23 inhibits viral RNA replication and synthesis of structural proteins. Normal cellular levels of SNAP23 are required for an early step in virus production, prior to or at the stage of virus RNA replication. We report that SNAP23 knockdown generates large, electron-light structures, and that infection of cells with these structures does not alter them, and those cells fail to generate viral RNA replication sites. We suggest that SNAP23 may play a role in maintaining membranes and lipids needed for generating virus replication organelles. Further investigation is needed to determine the precise role of this crucial SNARE protein in EV-D68 replication.

Indexed as

Enterovirus D, HumanCell LineCell MembraneMembrane FusionOrganellesQb-SNARE ProteinsQc-SNARE ProteinsVirus ReplicationQb-SNARE ProteinsQc-SNARE ProteinsSNAP23 protein, humanEV-D68PicornavirusSNAP23SNARE

Identifiers

PMID36527930
OpenAlexW4311159553

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.