Evidence map›Paper›PMID 36527735›Full record

ArticleAdvanced healthcare materials2023

Exploring the Potential of PEG-Heparin Hydrogels to Support Long-Term Ex Vivo Culture of Patient-Derived Breast Explant Tissues.

Maria K Koch, Akhilandeshwari Ravichandran, Berline Murekatete, Julien Clegg, Mary Teresa Joseph, Madison Hampson, Mitchell Jenkinson, Hannah S Bauer, Cameron Snell, Cheng Liu and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced healthcare materials, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Dynamic Hydrogels in Breast Tumor Models.Gels (Basel, Switzerland) · 2025
    Review
  5. Article
  6. Applications of Hydrogels in Emergency Therapy.Gels (Basel, Switzerland) · 2025
    Review
  7. Advanced Hydrogels in Breast Cancer Therapy.Gels (Basel, Switzerland) · 2024
    Review
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Maria K KochSchool of Mechanical, Medical and Process Engineering, Queensland University of Technology (QUT), Kelvin Grove, QLD, 4059, Australia.
Akhilandeshwari RavichandranSchool of Mechanical, Medical and Process Engineering, Queensland University of Technology (QUT), Kelvin Grove, QLD, 4059, Australia.
Berline MurekateteSchool of Mechanical, Medical and Process Engineering, Queensland University of Technology (QUT), Kelvin Grove, QLD, 4059, Australia.
Julien CleggSchool of Mechanical, Medical and Process Engineering, Queensland University of Technology (QUT), Kelvin Grove, QLD, 4059, Australia.
Mary Teresa JosephSchool of Mechanical, Medical and Process Engineering, Queensland University of Technology (QUT), Kelvin Grove, QLD, 4059, Australia.
Madison HampsonSchool of Mechanical, Medical and Process Engineering, Queensland University of Technology (QUT), Kelvin Grove, QLD, 4059, Australia.
Mitchell JenkinsonSchool of Mechanical, Medical and Process Engineering, Queensland University of Technology (QUT), Kelvin Grove, QLD, 4059, Australia.
Hannah S BauerSchool of Mechanical, Medical and Process Engineering, Queensland University of Technology (QUT), Kelvin Grove, QLD, 4059, Australia.
Cameron SnellPeter MacCallum Cancer Centre, Melbourne, VIC, 3000, Australia.
Cheng LiuMater Pathology, Mater Hospital Brisbane, Mater Health Services, Brisbane, QLD, 4101, Australia.
Madeline GoughMater Pathology, Mater Hospital Brisbane, Mater Health Services, Brisbane, QLD, 4101, Australia.
Erik W ThompsonCentre for the Personalised Analysis of Cancers, Queensland University of Technology (QUT), Translational Research Institute, Brisbane, QLD, 4102, Australia.
Carsten WernerLeibniz Institute of Polymer Research, 01069, Dresden, Germany.
Dietmar W HutmacherSchool of Mechanical, Medical and Process Engineering, Queensland University of Technology (QUT), Kelvin Grove, QLD, 4059, Australia.
Larisa M HauptSchool of Biomedical Sciences, Queensland University of Technology (QUT), Translational Research Institute, Brisbane, QLD, 4102, Australia.
Laura J BraySchool of Mechanical, Medical and Process Engineering, Queensland University of Technology (QUT), Kelvin Grove, QLD, 4059, Australia.ORCID 0000-0002-1174-0018

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Breast cancer is a complex, highly heterogenous, and dynamic disease and the leading cause of cancer-related death in women worldwide. Evaluation of the heterogeneity of breast cancer and its various subtypes is crucial to identify novel treatment strategies that can overcome the limitations of currently available options. Explant cultures of human mammary tissue have been known to provide important insights for the study of breast cancer structure and phenotype as they include the context of the surrounding microenvironment, allowing for the comprehensive exploration of patient heterogeneity. However, the major limitation of currently available techniques remains the short-term viability of the tissue owing to loss of structural integrity. Here, an ex vivo culture model using star-shaped poly(ethylene glycol) and maleimide-functionalized heparin (PEG-HM) hydrogels to provide structural support to the explant cultures is presented. The mechanical support allows the culture of the human mammary tissue for up to 3 weeks and prevent disintegration of the cellular structures including the epithelium and surrounding stromal tissue. Further, maintenance of epithelial phenotype and hormonal receptors is observed for up to 2 weeks of culture which makes them relevant for testing therapeutic interventions. Through this study, the importance of donor-to-donor variability and intra-patient tissue heterogeneity is reiterated.

Indexed as

Breast NeoplasmsHeparinBiocompatible MaterialsFemaleHumansHydrogelsPolyethylene GlycolsTumor MicroenvironmentBiocompatible MaterialsHeparinHydrogelsPolyethylene Glycolsbreast cancerex vivo cultureshydrogelspatient-derived explants

Identifiers

PMID36527735
PMCPMC11469079

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.