Evidence map›Paper›PMID 36527585›Full record

ArticleThe protein journal2023

Site-Directed Mutation of Salicylate Decarboxylase Gene and Mechanism of Ginkgo Acid Decarboxylation.

Yuanyuan Song, Yanying Hu, Jiaxin Li, Lin Wang, Wenjie Jing, Liming Zhang, Yujie Dai, Shiru Jia, Xuan Meng, Huitu Zhang

Abstract read
PubMed Publisher
In one paragraph

Article in The protein journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 79% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Yuanyuan Song *Tianjin Key Laboratory of Industrial Microbiology, Teda Campus, Tianjin University of Science and Technology, No. 9 of 13th Street, Tianjin Economic and Technological Development Zone TEDA, Tianjin, 300457, People's Republic of China.
Yanying Hu *Jining University, Xingtan Road, New District, Qufu City, Shandong Province, People's Republic of China.
Jiaxin LiTianjin Key Laboratory of Industrial Microbiology, Teda Campus, Tianjin University of Science and Technology, No. 9 of 13th Street, Tianjin Economic and Technological Development Zone TEDA, Tianjin, 300457, People's Republic of China.
Lin WangTianjin Key Laboratory of Industrial Microbiology, Teda Campus, Tianjin University of Science and Technology, No. 9 of 13th Street, Tianjin Economic and Technological Development Zone TEDA, Tianjin, 300457, People's Republic of China.
Wenjie JingTianjin Key Laboratory of Industrial Microbiology, Teda Campus, Tianjin University of Science and Technology, No. 9 of 13th Street, Tianjin Economic and Technological Development Zone TEDA, Tianjin, 300457, People's Republic of China.
Liming ZhangTianjin Key Laboratory of Industrial Microbiology, Teda Campus, Tianjin University of Science and Technology, No. 9 of 13th Street, Tianjin Economic and Technological Development Zone TEDA, Tianjin, 300457, People's Republic of China.
Yujie DaiTianjin Key Laboratory of Industrial Microbiology, Teda Campus, Tianjin University of Science and Technology, No. 9 of 13th Street, Tianjin Economic and Technological Development Zone TEDA, Tianjin, 300457, People's Republic of China. daiyujie@tust.edu.cn.ORCID 0000-0002-6484-4478
Shiru JiaTianjin Key Laboratory of Industrial Microbiology, Teda Campus, Tianjin University of Science and Technology, No. 9 of 13th Street, Tianjin Economic and Technological Development Zone TEDA, Tianjin, 300457, People's Republic of China.
Xuan MengTianjin Key Laboratory of Industrial Microbiology, Teda Campus, Tianjin University of Science and Technology, No. 9 of 13th Street, Tianjin Economic and Technological Development Zone TEDA, Tianjin, 300457, People's Republic of China.
Huitu ZhangTianjin Key Laboratory of Industrial Microbiology, Teda Campus, Tianjin University of Science and Technology, No. 9 of 13th Street, Tianjin Economic and Technological Development Zone TEDA, Tianjin, 300457, People's Republic of China.
Tianjin University of Science and Technology · CNJining University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ginkgo seed is an important Chinese medicine and food resource in China, but the toxicity of ginkgo acid in it limits its application. Previous studies have found that salicylic acid decarboxylase (Sdc) has a decarboxylation degradation effect on ginkgo acid. In order to improve the decarboxylation ability of Sdc to Ginkgo acid, 11 residues of the Sdc around the substrate (salicylic acid) were determined as mutation targets according to the analysis of crystal structure of Sdc (PDB ID:6JQX), from Trichosporon moniliiforme WU-0401, and a total of 30 single point mutant enzymes and one compound mutant enzyme were obtained. With Ginkgo acid C15:1 as the substrate, it was found from activity assay that Sdc-Y64T and Sdc-P191A had higher decarboxylation activity, which increased by 105.18% and 116.74% compared with that of wild type Sdc, respectively. The optimal pH for Sdc Y64T and Sdc-P191A to decarboxylate Ginkgo acid C15:1 was 5.5, which is the same as the wild type Sdc. The optimal temperature of Sdc-P191A was 50 °C, which was consistent with that of the wild type Sdc, but the optimal temperature of the mutant Sdc-Y64T was 40 °C, which was 10 °C lower than that of wild type Sdc.

Indexed as

Carboxy-LyasesGinkgo bilobaDecarboxylationMutationSalicylic AcidCarboxy-LyasesSalicylic AcidDecarboxylationGinkgo acidSalicylic acid decarboxylaseSite-directed mutation

Identifiers

PMID36527585
OpenAlexW4311822371

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.