ArticleBiotechnology for biofuels and bioproducts2022
Spatial-temporal regulation of fatty alcohol biosynthesis in yeast.
Article in Biotechnology for biofuels and bioproducts, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 22 citations in OpenAlex.
- Engineering metazoan fatty acid synthase to control chain length applied in yeast.Nature chemical biology · 2026Article
- Engineering robustSynthetic and systems biotechnology · 2025Article
- De novo biosynthesis of taxifolin in yeast peroxisomes.Microbial cell factories · 2025Article
- Advanced metabolic Engineering strategies for the sustainable production of free fatty acids and their derivatives using yeast.Journal of biological engineering · 2024Review
- Engineering Yeast Peroxisomes for α-Bisabolene Production from Sole Methanol with the Aid of Proteomic Analysis.JACS Au · 2024Article
- Promoter engineering enables precise metabolic regulation towards efficient β-elemene production inSynthetic and systems biotechnology · 2024Article
- Advances in the dynamic control of metabolic pathways inEngineering microbiology · 2023Review
- Peroxisomal metabolic coupling improves fatty alcohol production from sole methanol in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundConstruction of efficient microbial cell factories is one of the core steps for establishing green bio-manufacturing processes. However, the complex metabolic regulation makes it challenging in driving the metabolic flux toward the product biosynthesis. Dynamically coupling the biosynthetic pathways with the cellular metabolism at spatial-temporal manner should be helpful for improving the production with alleviating the cellular stresses.
resultsIn this study, we observed the mismatch between fatty alcohol biosynthesis and cellular metabolism, which compromised the fatty alcohol production in Saccharomyces cerevisiae. To enhance the fatty alcohol production, we spatial-temporally regulated fatty alcohol biosynthetic pathway by peroxisomal compartmentalization (spatial) and dynamic regulation of gene expression (temporal). In particular, fatty acid/acyl-CoA responsive promoters were identified by comparative transcriptional analysis, which helped to dynamically regulate the expression of acyl-CoA reductase gene MaFAR1 and improved fatty alcohol biosynthesis by 1.62-fold. Furthermore, enhancing the peroxisomal supply of acyl-CoA and NADPH further improved fatty alcohol production to 282 mg/L, 2.52 times higher than the starting strain.
conclusionsThis spatial-temporal regulation strategy partially coordinated fatty alcohol biosynthesis with cellular metabolism including peroxisome biogenesis and precursor supply, which should be applied for production of other products in microbes.
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