ArticleBMC biology2022
Construction and characterization of a genome-scale ordered mutant collection of Bacteroides thetaiotaomicron.
Article in BMC biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
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- Identification and characterization of the functional LolB ortholog inbioRxiv : the preprint server for biology · 2026Article
- Industrial and agricultural chemicals exhibit antimicrobial activity against human gut bacteria in vitro.Nature microbiology · 2025Article
- Bacterial fitness for plant colonization is influenced by plant growth substrate.The New phytologist · 2025Article
- Growth stage and interspecies interactions shape the cell biology and cell cycle characteristics of human gut bacteria Bacteroides thetaiotaomicron and Roseburia intestinalis.Communications biology · 2025Article
- Commensal gut bacteria employ de-chelatase HmuS to harvest iron from heme.The EMBO journal · 2025Article
- Review
- Arylsulfamates inhibit colonic Bacteroidota growth through a sulfatase-independent mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- A data-driven modeling framework for mapping genotypes to synthetic microbial community functions.bioRxiv : the preprint server for biology · 2025Article
- Heme and iron limitation in a GI-tract foundation species leads to a reshuffling of the metalloproteome and a shift toward manganese usage.Frontiers in chemistry · 2025Article
- Simvastatin induces human gut bacterial cell surface genes.Molecular microbiology · 2024Article
- CRISPRi functional genomics in bacteria and its application to medical and industrial research.Microbiology and molecular biology reviews : MMBR · 2024Review
- High-throughput functional genomics: A (myco)bacterial perspective.Molecular microbiology · 2023Review
- Construction and characterization of a genome-scale ordered mutant collection of Bacteroides thetaiotaomicron.BMC biology · 2022Article
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Authors and funding
16 authors.
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Abstract
backgroundOrdered transposon-insertion collections, in which specific transposon-insertion mutants are stored as monocultures in a genome-scale collection, represent a promising tool for genetic dissection of human gut microbiota members. However, publicly available collections are scarce and the construction methodology remains in early stages of development.
resultsHere, we describe the assembly of a genome-scale ordered collection of transposon-insertion mutants in the model gut anaerobe Bacteroides thetaiotaomicron VPI-5482 that we created as a resource for the research community. We used flow cytometry to sort single cells from a pooled library, located mutants within this initial progenitor collection by applying a pooling strategy with barcode sequencing, and re-arrayed specific mutants to create a condensed collection with single-insertion strains covering >2500 genes. To demonstrate the potential of the condensed collection for phenotypic screening, we analyzed growth dynamics and cell morphology. We identified both growth defects and altered cell shape in mutants disrupting sphingolipid synthesis and thiamine scavenging. Finally, we analyzed the process of assembling the B. theta condensed collection to identify inefficiencies that limited coverage. We demonstrate as part of this analysis that the process of assembling an ordered collection can be accurately modeled using barcode sequencing data.
conclusionWe expect that utilization of this ordered collection will accelerate research into B. theta physiology and that lessons learned while assembling the collection will inform future efforts to assemble ordered mutant collections for an increasing number of gut microbiota members.
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