Evidence map›Paper›PMID 36526429›Full record

ArticleNeurology2023

Detection of SARS-CoV-2 Nucleocapsid and Microvascular Disease in the Brain: A Case Report.

Catherine DeMarino, Myoung-Hwa Lee, Maria Cowen, Joseph P Steiner, Sara Inati, Ashish H Shah, Kareem A Zaghloul, Avindra Nath

Open access · hybridAbstract readCase Reports
PubMed Full text at PMC Publisher
In one paragraph

Article in Neurology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.7field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 15 citations in OpenAlex.

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  3. A survey of SARS-CoV-2 tropism.Infectious diseases & immunity · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Catherine DeMarinoFrom the Section for Infections of the Nervous System (C.D., M.-H.L., M.C., A.N.), NeuroTherapeutics Development Unit (J.P.S.), Translational Neuroscience Center, Neurophysiology of Epilepsy Unit (S.I.), and Surgical Neurology Branch (A.H.S., K.A.Z.), National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD.
Myoung-Hwa LeeFrom the Section for Infections of the Nervous System (C.D., M.-H.L., M.C., A.N.), NeuroTherapeutics Development Unit (J.P.S.), Translational Neuroscience Center, Neurophysiology of Epilepsy Unit (S.I.), and Surgical Neurology Branch (A.H.S., K.A.Z.), National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD.
Maria CowenFrom the Section for Infections of the Nervous System (C.D., M.-H.L., M.C., A.N.), NeuroTherapeutics Development Unit (J.P.S.), Translational Neuroscience Center, Neurophysiology of Epilepsy Unit (S.I.), and Surgical Neurology Branch (A.H.S., K.A.Z.), National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD.
Joseph P SteinerFrom the Section for Infections of the Nervous System (C.D., M.-H.L., M.C., A.N.), NeuroTherapeutics Development Unit (J.P.S.), Translational Neuroscience Center, Neurophysiology of Epilepsy Unit (S.I.), and Surgical Neurology Branch (A.H.S., K.A.Z.), National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD.
Sara InatiFrom the Section for Infections of the Nervous System (C.D., M.-H.L., M.C., A.N.), NeuroTherapeutics Development Unit (J.P.S.), Translational Neuroscience Center, Neurophysiology of Epilepsy Unit (S.I.), and Surgical Neurology Branch (A.H.S., K.A.Z.), National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD.
Ashish H ShahFrom the Section for Infections of the Nervous System (C.D., M.-H.L., M.C., A.N.), NeuroTherapeutics Development Unit (J.P.S.), Translational Neuroscience Center, Neurophysiology of Epilepsy Unit (S.I.), and Surgical Neurology Branch (A.H.S., K.A.Z.), National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD.
Kareem A ZaghloulFrom the Section for Infections of the Nervous System (C.D., M.-H.L., M.C., A.N.), NeuroTherapeutics Development Unit (J.P.S.), Translational Neuroscience Center, Neurophysiology of Epilepsy Unit (S.I.), and Surgical Neurology Branch (A.H.S., K.A.Z.), National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD.
Avindra NathFrom the Section for Infections of the Nervous System (C.D., M.-H.L., M.C., A.N.), NeuroTherapeutics Development Unit (J.P.S.), Translational Neuroscience Center, Neurophysiology of Epilepsy Unit (S.I.), and Surgical Neurology Branch (A.H.S., K.A.Z.), National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD. natha@ninds.nih.gov.ORCID 0000-0003-0927-5855
National Institute of Neurological Disorders and Stroke · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

objectivesSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can cause a wide range of neurologic complications; however, its neuropenetrance during the acute phase of the illness is unknown.

methodsExtracellular vesicles were isolated from brain biopsy tissue from a patient undergoing epilepsy surgery using ultracentrifugation and analyzed by Western blot and qPCR for the presence of virus protein and RNA, respectively. Biopsy tissue was assessed by immunohistochemistry for the presence of microvascular damage and compared with 3 other non-COVID surgical epilepsy brain tissues.

resultsWe demonstrate the presence of viral nucleocapsid protein in extracellular vesicles and microvascular disease in the brain of a patient undergoing epilepsy surgery shortly after SARS-CoV-2 infection. Endothelial cell activation was indicated by increased levels of platelet endothelial cell adhesion molecule-1 and was associated with fibrinogen leakage and immune cell infiltration in the biopsy tissue as compared with control non-COVID surgical epilepsy brain tissues. DISCUSSION: Despite the lack of evidence of viral replication within the brain, the presence of the nucleocapsid protein was associated with disease-specific endothelial cell activation, fibrinogen leakage, and immune cell infiltration.

Indexed as

COVID-19SARS-CoV-2BrainCoronavirus Nucleocapsid ProteinsHumansNucleocapsidNucleocapsid ProteinsCoronavirus Nucleocapsid ProteinsNucleocapsid Proteins

Identifiers

PMID36526429
PMCPMC10065204
OpenAlexW4311824222

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.