ArticleeLife2022
Single-cell RNA sequencing and lineage tracing confirm mesenchyme to epithelial transformation (MET) contributes to repair of the endometrium at menstruation.
Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 51 citations in OpenAlex.
- Engineering the human endometrium at the intersection of development and reproduction.Nature biomedical engineering · 2026Review
- Endometrial cells with high ALDH activity contribute to uterine development and regeneration.eLife · 2026Article
- Endometrial cells with high ALDH activity contribute to uterine development and regeneration.Research square · 2026Article
- Mesenchymal-epithelial transition supports rapid repair of the endometrial epithelium during postpartum uterine regeneration.Communications biology · 2026Article
- Article
- Luminal epithelium remodeling underlies endometrial regeneration during menstruation and pregnancy.bioRxiv : the preprint server for biology · 2026Article
- In preprints: shedding light - chemogenetic induction of menstruation in mice.Development (Cambridge, England) · 2026Article
- Uterine closure after cesarean delivery: surgical technique, biological rationale, and clinical implications.American journal of obstetrics and gynecology · 2026Review
- Site-specific ERα phosphorylation determines sex-dependent metabolic, reproductive, and body-compositional phenotypes in mice.iScience · 2025Article
- Mesenchymal-to-epithelial transition of perivascular cells contributes to endometrial re-epithelialization.Nature communications · 2025Article
- Induction of menstruation in mice reveals the regulation of menstrual shedding.bioRxiv : the preprint server for biology · 2025Article
- Unlocking the Secrets of the Endometrium: Stem Cells, Niches and Modern Methodologies.Biomedicines · 2025Review
- Single-cell RNA sequencing identifies the prolactin receptor as a therapeutic target in adenomyosis.Signal transduction and targeted therapy · 2025Article
- A mouse model engineered to spatiotemporally control Cre expression in progesterone receptor positive cells†.Biology of reproduction · 2025Article
- S100A8 regulated by estrogen improves injured endometrial epithelium reconstruction by promoting tight junction formation and stromal cell transformation.Scientific reports · 2025Article
- Comprehensive single-cell transcriptome analysis of autologous platelet-rich plasma therapy on human thin endometrium.Scientific reports · 2025Article
- Revolutionizing Implantation Studies: Uterine-Specific Models and Advanced Technologies.Biomolecules · 2025Review
- Multi-omics-based mapping of decidualization resistance in patients with a history of severe preeclampsia.Nature medicine · 2025Article
- Dynamic roles of ILC3 in endometrial repair and regeneration.Discovery immunology · 2025Article
- Surface Molecular Markers for the Isolation of Viable Fibroblast Subpopulations in the Female Reproductive Tract: A Comprehensive Review.International journal of molecular sciences · 2024Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The human endometrium experiences repetitive cycles of tissue wounding characterised by piecemeal shedding of the surface epithelium and rapid restoration of tissue homeostasis. In this study, we used a mouse model of endometrial repair and three transgenic lines of mice to investigate whether epithelial cells that become incorporated into the newly formed luminal epithelium have their origins in one or more of the mesenchymal cell types present in the stromal compartment of the endometrium. Using scRNAseq, we identified a novel population of PDGFRb + mesenchymal stromal cells that developed a unique transcriptomic signature in response to endometrial breakdown/repair. These cells expressed genes usually considered specific to epithelial cells and in silico trajectory analysis suggested they were stromal fibroblasts in transition to becoming epithelial cells. To confirm our hypothesis we used a lineage tracing strategy to compare the fate of stromal fibroblasts (PDGFRa+) and stromal perivascular cells (NG2/CSPG4+). We demonstrated that stromal fibroblasts can undergo a mesenchyme to epithelial transformation and become incorporated into the re-epithelialised luminal surface of the repaired tissue. This study is the first to discover a novel population of wound-responsive, plastic endometrial stromal fibroblasts that contribute to the rapid restoration of an intact luminal epithelium during endometrial repair. These findings form a platform for comparisons both to endometrial pathologies which involve a fibrotic response (Asherman's syndrome, endometriosis) as well as other mucosal tissues which have a variable response to wounding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.