Evidence map›Paper›PMID 36522651›Full record

ArticleGenome biology2022

Automated assembly scaffolding using RagTag elevates a new tomato system for high-throughput genome editing.

Michael Alonge, Ludivine Lebeigle, Melanie Kirsche, Katie Jenike, Shujun Ou, Sergey Aganezov, Xingang Wang, Zachary B Lippman, Michael C Schatz, Sebastian Soyk

Abstract read
In one paragraph

Article in Genome biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 672 papers.

0numbers the graph read from it
0cells of the map it votes in
672citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

672 citing papers in PubMed.

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  3. 'International journal of systematic and evolutionary microbiology · 2026
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  16. Pangenome ofEcology and evolution · 2026
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612 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Michael AlongeDepartment of Computer Science, Johns Hopkins University, Baltimore, MD, 21218, USA.
Ludivine LebeigleCenter for Integrative Genomics, University of Lausanne, CH-1015, Lausanne, Switzerland.
Melanie KirscheDepartment of Computer Science, Johns Hopkins University, Baltimore, MD, 21218, USA.
Katie JenikeDepartment of Computer Science, Johns Hopkins University, Baltimore, MD, 21218, USA.
Shujun OuDepartment of Computer Science, Johns Hopkins University, Baltimore, MD, 21218, USA.
Sergey AganezovDepartment of Computer Science, Johns Hopkins University, Baltimore, MD, 21218, USA.
Xingang WangCold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724, USA.
Zachary B LippmanCold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724, USA.
Michael C SchatzDepartment of Computer Science, Johns Hopkins University, Baltimore, MD, 21218, USA. mschatz@cs.jhu.edu.
Sebastian SoykCenter for Integrative Genomics, University of Lausanne, CH-1015, Lausanne, Switzerland. sebastian.soyk@unil.ch.ORCID 0000-0003-0325-4404

Funding

Single-Cell Biology Shared ResourceP30CA045508 · NCI · COLD SPRING HARBOR LABORATORY · PI John Edward Moses · 1987 to 2026
$118.9M
Graphical Processing Units and a Large-Memory Compute Node for Applications in Genomics, Neuroscience, and Structural BiologyS10OD028632 · OD · COLD SPRING HARBOR LABORATORY · PI SIEPEL, ADAM CHARLES · 2020 to 2020
$437k
Howard Hughes Medical InstituteNCI NIH HHS P30 CA045508NIH HHS S10 OD028632
6 · The paper itself

Abstract

Advancing crop genomics requires efficient genetic systems enabled by high-quality personalized genome assemblies. Here, we introduce RagTag, a toolset for automating assembly scaffolding and patching, and we establish chromosome-scale reference genomes for the widely used tomato genotype M82 along with Sweet-100, a new rapid-cycling genotype that we developed to accelerate functional genomics and genome editing in tomato. This work outlines strategies to rapidly expand genetic systems and genomic resources in other plant species.

Indexed as

Solanum lycopersicumGene EditingGenomeGenomicsGenotypeAssembly scaffoldingGenome editingGenome sequencingTomato

Identifiers

PMID36522651
PMCPMC9753292

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.