ArticleNature chemical biology2023
De novo engineering of a bacterial lifestyle program.
Article in Nature chemical biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed.
- Gene circuit-based sensors.Fundamental research · 2025Review
- A trans-acting sRNA SaaS targeting hilD, cheA and csgA to inhibit biofilm formation of S. Enteritidis.Journal of advanced research · 2025Article
- Developing Quorum Sensing-Based Collaborative Dynamic Control System in Halomonas TD01.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Engineering biology applications for environmental solutions: potential and challenges.Nature communications · 2025Review
- Combating oral biofilms in Nigerian schoolchildren: a synergistic approach using Macrosphyra longistyla extracts and titanium-ferrite nanoparticles.GMS hygiene and infection control · 2025Article
- How bacteria actively use passive physics to make biofilms.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Diurnal switches in diazotrophic lifestyle increase nitrogen contribution to cereals.Nature communications · 2023Article
- A change of phase.Nature chemical biology · 2023Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synthetic biology has shown remarkable potential to program living microorganisms for applications. However, a notable discrepancy exists between the current engineering practice-which focuses predominantly on planktonic cells-and the ubiquitous observation of microbes in nature that constantly alternate their lifestyles on environmental variations. Here we present the de novo construction of a synthetic genetic program that regulates bacterial life cycle and enables phase-specific gene expression. The program is orthogonal, harnessing an engineered protein from 45 candidates as the biofilm matrix building block. It is also highly controllable, allowing directed biofilm assembly and decomposition as well as responsive autonomous planktonic-biofilm phase transition. Coupling to synthesis modules, it is further programmable for various functional realizations that conjugate phase-specific biomolecular production with lifestyle alteration. This work establishes a versatile platform for microbial engineering across physiological regimes, thereby shedding light on a promising path for gene circuit applications in complex contexts.
Indexed as
Identifiers
36522463What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.