Evidence map›Paper›PMID 36521929›Full record

ArticleJournal for immunotherapy of cancer2022

Activated NK cells reprogram MDSCs via NKG2D-NKG2DL and IFN-γ to modulate antitumor T-cell response after cryo-thermal therapy.

Peng Peng, Yue Lou, Shicheng Wang, Junjun Wang, Zelu Zhang, Peishan Du, Jiamin Zheng, Ping Liu, Lisa X Xu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal for immunotherapy of cancer, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
2.8field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 38 citations in OpenAlex.

  1. Article
  2. Review
  3. NKG2A inhibition promotes NK cell-CD8Nature communications · 2026
    Article
  4. Review
  5. Review
  6. Article
  7. Review
  8. Article
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  10. Review
  11. Article
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  14. Article
  15. Review
  16. Article
  17. Article
  18. Frontiers in pharmacology · 2025
    Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Peng PengSchool of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.ORCID 0000-0002-8341-3008
Yue LouSchool of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.
Shicheng WangSchool of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.
Junjun WangSchool of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.
Zelu ZhangSchool of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.
Peishan DuSchool of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.
Jiamin ZhengSchool of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.
Ping LiuSchool of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China lisaxu@sjtu.edu.cn pingliu@sjtu.edu.cn.
Lisa X XuSchool of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China lisaxu@sjtu.edu.cn pingliu@sjtu.edu.cn.
Shanghai Jiao Tong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMyeloid-derived suppressor cells (MDSCs) can potently inhibit T-cell activity, promote growth and metastasis of tumor and contribute to resistance to immunotherapy. Targeting MDSCs to alleviate their protumor functions and immunosuppressive activities is intimately associated with cancer immunotherapy. Natural killer (NK) cells can engage in crosstalk with multiple myeloid cells to alter adaptive immune responses, triggering T-cell immunity. However, whether the NK-cell-MDSC interaction can modulate the T-cell immune response requires further study. Cryo-thermal therapy could induce the maturation of MDSCs by creating an acute inflammatory environment to elicit a CD4

methodsNK cells were depleted and NKG2D was blocked with monoclonal antibodies in vivo. MDSCs, NK cells and T cells were assessed by flow cytometry and isolated by magnetic-activated cell sorting (MACS). MDSCs and NK cells were cocultured with T cells to determine their immunological function. The transcriptional profiles of MDSCs were measured by qRT-PCR and RNA-sequencing. Isolated NK cells and MDSCs by MACS were cocultured to study the viability and maturation of MDSCs regulated by NK cells. TIMER was used to comprehensively examine the immunological, clinical, and genomic features of tumors.

resultsNK-cell activation after cryo-thermal therapy decreased MDSC accumulation and reprogrammed immunosuppressive MDSCs toward a mature phenotype to promote T cell antitumor immunity. Furthermore, we discovered that NK cells could kill MDSCs via the NKG2D-NKG2DL axis and promote MDSC maturation by interferon gamma (IFN-γ) in response to NKG2D. In addition, CD4

conclusionsCryo-thermal therapy induces effective CD4

Indexed as

Myeloid-Derived Suppressor CellsNeoplasmsHumansInterferon-gammaKiller Cells, NaturalLymphocyte ActivationNK Cell Lectin-Like Receptor Subfamily KT-LymphocytesInterferon-gammaNK Cell Lectin-Like Receptor Subfamily KAdaptive ImmunityCD4-Positive T-LymphocytesImmunity, InnateKiller Cells, NaturalMyeloid-Derived Suppressor Cells

Identifiers

PMID36521929
PMCPMC9756281
OpenAlexW4311598033

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.