Evidence map›Paper›PMID 36520355›Full record

ArticleMarine biotechnology (New York, N.Y.)2023

Transcriptomes of Testes at Different Developmental Stages in the Opsariichthys bidens Predict Key Genes for Testis Development and Spermatogenesis.

Jie Ding, Daojun Tang, Yibo Zhang, Xinming Gao, Chen Du, Weiliang Shen, Shan Jin, Junquan Zhu

Abstract read
PubMed Publisher
In one paragraph

Article in Marine biotechnology (New York, N.Y.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.5field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Development of a Sex-Specific Marker for the Chinese Hooksnout CarpAnimals : an open access journal from MDPI · 2025
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Jie Ding *Key Laboratory of Applied Marine Biotechnology of Ministry of Education, College of Marine Sciences, Ningbo University, Ningbo, 315832, Zhejiang, China.
Daojun Tang *Key Laboratory of Applied Marine Biotechnology of Ministry of Education, College of Marine Sciences, Ningbo University, Ningbo, 315832, Zhejiang, China.
Yibo ZhangKey Laboratory of Applied Marine Biotechnology of Ministry of Education, College of Marine Sciences, Ningbo University, Ningbo, 315832, Zhejiang, China.
Xinming GaoKey Laboratory of Applied Marine Biotechnology of Ministry of Education, College of Marine Sciences, Ningbo University, Ningbo, 315832, Zhejiang, China.
Chen DuKey Laboratory of Applied Marine Biotechnology of Ministry of Education, College of Marine Sciences, Ningbo University, Ningbo, 315832, Zhejiang, China.
Weiliang ShenNingbo Academy of Oceanology and Fishery, Ningbo, 315103, Zhejiang, China.
Shan JinKey Laboratory of Applied Marine Biotechnology of Ministry of Education, College of Marine Sciences, Ningbo University, Ningbo, 315832, Zhejiang, China.
Junquan ZhuKey Laboratory of Applied Marine Biotechnology of Ministry of Education, College of Marine Sciences, Ningbo University, Ningbo, 315832, Zhejiang, China. zhujunquan@nbu.edu.cn.
Ningbo University · CNNingbo Academy of Agricultural Sciences · CN

Funding

Ningbo Science and Technology Plan Projects 2019C10057Science and Technology Planning Programs of Zhejiang Province 2020C02014, 2016C32062the National Natural Science Foundation of China (NSFC)-Zhejiang Joint Fund for the Integration of Industrialisation and Informatisation U1809212
6 · The paper itself

Abstract

Testis development is a complex process involving multiple genes, and the molecular mechanisms underlying testis development in Opsariichthys bidens remain unclear. We performed transcriptome sequencing analysis on a total of 12 samples of testes from stages II, III, IV, and V of O. bidens and obtained a total of 79.52 Gb clean data, as well as 288,573 transcripts and 116,215 unigenes. Differential expression analysis showed that 22,857 differentially expressed genes (DEGs) were screened in six comparison groups (III vs. II, IV vs. II, V vs. II, IV vs. III, V vs. III, and V vs. IV). Kyoto Encyclopedia of Genes and Genomes enrichment analysis of DEGs showed that six comparison groups were significantly enriched for a total of 20 significantly up- or down-regulated pathways, including six pathways related to signal transduction, three pathways related to energy metabolism, five pathways related to disease, and two pathways related to ribosomes. Furthermore, our investigation revealed that DEGs were enriched in several important functional pathways, such as Huntington's disease signaling pathway, TGF-β signaling pathway, and ribosome signaling pathway. Protein-protein interaction network analysis of DEGs identified 63 up-regulated hub genes, including 9 kinesin genes and 2 cytoplasmic dynein genes, and 39 down-regulated hub genes, including 13 ribosomal protein genes. This result contributes to the knowledge of spermatogenesis and testis development in O. bidens.

Indexed as

TestisTranscriptomeComputational BiologyGene Expression ProfilingHumansMaleSpermatogenesisHub geneOpsariichthys bidensTestis developmentTranscriptome

Identifiers

PMID36520355
OpenAlexW4311582299

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.