Evidence map›Paper›PMID 36519469›Full record

ArticleArteriosclerosis, thrombosis, and vascular biology2023

Bicuspid Aortic Valve-Associated Regulatory Regions Reveal

Tingting Huang, Jiaxi Cheng, Hao Feng, Wei Zhou, Ping Qiu, Dong Zhou, Dongshan Yang, Jifeng Zhang, Cristen Willer, Y Eugene Chen and 2 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Arteriosclerosis, thrombosis, and vascular biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
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  3. Tanshinone IIChinese herbal medicines · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 2 countries.

Tingting Huang *Department of Cardiac Surgery (T.H., J.C., H.F., P.Q., D.Z., Y.E.C., D.M., B.Y.), University of Michigan, Ann Arbor.
Jiaxi Cheng *Department of Cardiac Surgery (T.H., J.C., H.F., P.Q., D.Z., Y.E.C., D.M., B.Y.), University of Michigan, Ann Arbor.
Hao FengDepartment of Cardiac Surgery (T.H., J.C., H.F., P.Q., D.Z., Y.E.C., D.M., B.Y.), University of Michigan, Ann Arbor.
Wei ZhouAnalytic and Translational Genetics Unit, Massachusetts General Hospital, Boston (W.Z.).
Ping QiuDepartment of Cardiac Surgery (T.H., J.C., H.F., P.Q., D.Z., Y.E.C., D.M., B.Y.), University of Michigan, Ann Arbor.
Dong ZhouDepartment of Cardiac Surgery (T.H., J.C., H.F., P.Q., D.Z., Y.E.C., D.M., B.Y.), University of Michigan, Ann Arbor.
Dongshan YangDepartment of Internal Medicine (D.Y., J.Z., C.W., Y.E.C.), University of Michigan, Ann Arbor.
Jifeng ZhangDepartment of Internal Medicine (D.Y., J.Z., C.W., Y.E.C.), University of Michigan, Ann Arbor.ORCID 0000-0001-5161-4705
Cristen WillerDepartment of Internal Medicine (D.Y., J.Z., C.W., Y.E.C.), University of Michigan, Ann Arbor.ORCID 0000-0001-5645-4966
Y Eugene ChenDepartment of Cardiac Surgery (T.H., J.C., H.F., P.Q., D.Z., Y.E.C., D.M., B.Y.), University of Michigan, Ann Arbor.ORCID 0000-0003-2357-7825
Dogukan MizrakDepartment of Cardiac Surgery (T.H., J.C., H.F., P.Q., D.Z., Y.E.C., D.M., B.Y.), University of Michigan, Ann Arbor.ORCID 0000-0002-9885-9686
Bo YangDepartment of Cardiac Surgery (T.H., J.C., H.F., P.Q., D.Z., Y.E.C., D.M., B.Y.), University of Michigan, Ann Arbor.ORCID 0000-0002-2158-9155
University of Michigan · USCentral South University · CNIHI Corporation (United States) · USMassachusetts General Hospital · US

Funding

Using genetics to discover mechanisms of myocardial infarctionR01HL109946 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI YUQING Eugene CHEN, Ida Liisa Surakka · 2011 to 2026
$8.9M
Therapeutic Insights into Loeys-Dietz Syndrome using Humanized ModelsR01HL151776 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Bo Yang · 2020 to 2026
$4.2M
Define the mechanisms of aortopathy in bicuspid aortic valve patientsR01HL141891 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI YANG, BO · 2018 to 2022
$3.5M
Development of gene editing based therapy for cardiovascular diseasesR01HL159900 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI CHEN, YUQING EUGENE, HAN, RENZHI · 2021 to 2024
$2.8M
BAF60c and abdominal aortic aneurysmR01HL153710 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ZHANG, JIFENG · 2021 to 2025
$2.7M
Browning of perivascular adipose tissue protects against thoracic aortic aneurysmR01HL159871 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI CHEN, YUQING EUGENE · 2022 to 2025
$2.3M
Kruppel-like factor 11 (KLF11) and atherosclerosisR01HL138139 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ZHANG, JIFENG · 2017 to 2021
$2.3M
Novel methods to improve nuclease mediated homologous recombination, Administrative supplR42GM122181 · NIGMS · ATGC, INC. · PI ZHANG, JIFENG · 2022 to 2023
$2.1M
Effect of mutations in the TGF-beta pathway genes on smooth muscle cell differentiation using patient derived iPSCs.K08HL130614 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI YANG, BO · 2016 to 2020
$848k
Novel methods to improve nuclease mediated homologous recombinationR41GM122181 · NIGMS · ATGC, INC. · PI ZHANG, JIFENG · 2017 to 2017
$225k
NHLBI NIH HHS K08 HL130614NHLBI NIH HHS R01 HL109946NHLBI NIH HHS R01 HL138139NHLBI NIH HHS R01 HL141891NHLBI NIH HHS R01 HL151776NHLBI NIH HHS R01 HL153710NHLBI NIH HHS R01 HL159871NHLBI NIH HHS R01 HL159900NIGMS NIH HHS R41 GM122181NIGMS NIH HHS R42 GM122181
6 · The paper itself

Abstract

backgroundThe endothelial-mesenchymal transition (EndoMT) is a fundamental process for heart valve formation and defects in EndoMT cause aortic valve abnormalities. Our previous genome-wide association study identified multiple variants in a large chromosome 8 segment as significantly associated with bicuspid aortic valve (BAV). The objective of this study is to determine the biological effects of this large noncoding segment in human induced pluripotent stem cell (hiPSC)-based EndoMT.

methodsA large genomic segment enriched for BAV-associated variants was deleted in hiPSCs using 2-step CRISPR/Cas9 editing. To address the effects of the variants on

resultsThe large deletion impaired hiPSC-based EndoMT in multiple biallelic clones compared with their isogenic control. It also reduced

conclusionsBAV-associated distal regions regulate

Indexed as

Bicuspid Aortic Valve DiseaseHeart Valve DiseasesInduced Pluripotent Stem CellsAortic ValveEndothelial CellsGATA4 Transcription FactorGenome-Wide Association StudyHumansRegulatory Sequences, Nucleic AcidGATA4 protein, humanGATA4 Transcription Factorbicuspid aortic valveCRISPR-Cas systemsGATA4 transcription factorheart defectsinduced pluripotent stem cells

Identifiers

PMID36519469
PMCPMC10038164
OpenAlexW4311582206

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.