Evidence map›Paper›PMID 36519269›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2023

Defined Microenvironments Trigger In Vitro Gastrulation in Human Pluripotent Stem Cells.

Pallavi Srivastava, Sara Romanazzo, Chantal Kopecky, Stephanie Nemec, Jake Ireland, Thomas G Molley, Kang Lin, Pavithra B Jayathilaka, Elvis Pandzic, Avani Yeola and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 21 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
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  5. Article
  6. Advancing Synthetic Hydrogels through Nature-Inspired Materials Chemistry.Advanced materials (Deerfield Beach, Fla.) · 2024
    Review
  7. Article
  8. Article
  9. Review
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  11. Article
  12. Defined Microenvironments Trigger In Vitro Gastrulation in Human Pluripotent Stem Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 1 country.

Pallavi SrivastavaSchool of Chemistry, Australian Centre for NanoMedicine, University of New South Wales, Sydney, NSW, 2052, Australia.ORCID 0000-0003-4322-2466
Sara RomanazzoSchool of Chemistry, Australian Centre for NanoMedicine, University of New South Wales, Sydney, NSW, 2052, Australia.
Chantal KopeckySchool of Chemistry, Australian Centre for NanoMedicine, University of New South Wales, Sydney, NSW, 2052, Australia.
Stephanie NemecSchool of Materials Science and Engineering, University of New South Wales, Sydney, NSW, 2052, Australia.
Jake IrelandSchool of Chemistry, Australian Centre for NanoMedicine, University of New South Wales, Sydney, NSW, 2052, Australia.
Thomas G MolleySchool of Materials Science and Engineering, University of New South Wales, Sydney, NSW, 2052, Australia.
Kang LinSchool of Materials Science and Engineering, University of New South Wales, Sydney, NSW, 2052, Australia.
Pavithra B JayathilakaSchool of Chemistry, Australian Centre for NanoMedicine, University of New South Wales, Sydney, NSW, 2052, Australia.
Elvis PandzicKatharina Gaus Light Microscopy Facility, Mark Wainwright Analytical Centre, University of New South Wales, Sydney, NSW, 2052, Australia.
Avani YeolaAdult Cancer Program, School of Clinical Medicine, Lowy Cancer Research Centre, UNSW Sydney, Sydney, NSW, 2052, Australia.ORCID 0000-0002-6718-0283
Vashe ChandrakanthanSchool of Biomedical Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.
John PimandaSchool of Biomedical Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.ORCID 0000-0002-0509-8962
Kristopher KilianSchool of Chemistry, Australian Centre for NanoMedicine, University of New South Wales, Sydney, NSW, 2052, Australia.ORCID 0000-0002-8963-9796
UNSW Sydney · AUCancer Institute of New South Wales · AU

Funding

Engineered microtumor arrays for development of combination therapiesR01CA251443 · NCI · UNIVERSITY OF NEW SOUTH WALES · PI COPLAND, JOHN A., KILIAN, KRISTOPHER ALAN · 2020 to 2024
$998k
NCI NIH HHS R01 CA251443NIH HHS R01CA251443
6 · The paper itself

Abstract

Gastrulation is a stage in embryo development where three germ layers arise to dictate the human body plan. In vitro models of gastrulation have been demonstrated by treating pluripotent stem cells with soluble morphogens to trigger differentiation. However, in vivo gastrulation is a multistage process coordinated through feedback between soluble gradients and biophysical forces, with the multipotent epiblast transforming to the primitive streak followed by germ layer segregation. Here, the authors show how constraining pluripotent stem cells to hydrogel islands triggers morphogenesis that mirrors the stages preceding in vivo gastrulation, without the need for exogenous supplements. Within hours of initial seeding, cells display a contractile phenotype at the boundary, which leads to enhanced proliferation, yes-associated protein (YAP) translocation, epithelial to mesenchymal transition, and emergence of SRY-box transcription factor 17 (SOX17)

Indexed as

Cellular MicroenvironmentGastrulationPluripotent Stem CellsEpithelial-Mesenchymal TransitionGerm LayersHumansMechanotransduction, CellularSOXF Transcription FactorsYAP-Signaling ProteinsSOX17 protein, humanSOXF Transcription FactorsYAP-Signaling Proteinsembryogenesisgastrulationhydrogelmicropatterningpluripotent stem cells

Identifiers

PMID36519269
PMCPMC9929265
OpenAlexW4311515970

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.