Evidence map›Paper›PMID 36518493›Full record

ArticleFrontiers in plant science2022

An integrated physiology, cytology, and proteomics analysis reveals a network of sugarcane protoplast responses to enzymolysis.

Demei Zhang, Rui Wang, Jiming Xiao, Shuifang Zhu, Xinzhu Li, Shijian Han, Zhigang Li, Yang Zhao, M J I Shohag, Zhenli He and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in plant science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Review
  3. Modifying the Expression of Cysteine Protease GeneInternational journal of molecular sciences · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Demei ZhangGuangxi Key Laboratory of Sugarcane Biology, College of Agriculture, Guangxi University, Nanning, China.
Rui WangGuangxi Key Laboratory of Sugarcane Biology, College of Agriculture, Guangxi University, Nanning, China.
Jiming XiaoGuangxi Key Laboratory of Sugarcane Biology, College of Agriculture, Guangxi University, Nanning, China.
Shuifang ZhuGuangxi Key Laboratory of Sugarcane Biology, College of Agriculture, Guangxi University, Nanning, China.
Xinzhu LiSchool of Biomedical Engineering, South-Central Minzu University, Wuhan, China.
Shijian HanGuangxi Key Laboratory of Sugarcane Biology, College of Agriculture, Guangxi University, Nanning, China.
Zhigang LiGuangxi Key Laboratory of Sugarcane Biology, College of Agriculture, Guangxi University, Nanning, China.
Yang ZhaoGuangxi Key Laboratory of Sugarcane Biology, College of Agriculture, Guangxi University, Nanning, China.
M J I ShohagInstitute of Food and Agricultural Sciences (IFAS) Indian River Research and Education Center, University of Florida, Fort Pierce, FL, United States.
Zhenli HeInstitute of Food and Agricultural Sciences (IFAS) Indian River Research and Education Center, University of Florida, Fort Pierce, FL, United States.
Suli LiGuangxi Key Laboratory of Sugarcane Biology, College of Agriculture, Guangxi University, Nanning, China.
Guangxi University · CNIndian River State College · USMinzu University of China · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The protoplast experimental system eis an effective tool for functional genomics and cell fusion breeding. However, the physiological and molecular mechanisms of protoplast response to enzymolysis are not clear, which has become a major obstacle to protoplast regeneration. Here, we used physiological, cytological, proteomics and gene expression analysis to compare the young leaves of sugarcane and enzymolized protoplasts. After enzymatic digestion, we obtained protoplasts with viability of > 90%. Meanwhile, the content of malondialdehyde, an oxidation product, increased in the protoplasts following enzymolysis, and the activity of antioxidant enzymes, such as peroxidase (POD), catalase (CAT), acid peroxidase (APX), and O

Indexed as

cytologyenzymolysisoxidative stressproteomicsprotoplastsugarcane

Identifiers

PMID36518493
PMCPMC9744229
OpenAlexW4310119587

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.