ArticleBiotechnology for biofuels and bioproducts2022
Metabolic engineering of Corynebacterium glutamicum for acetate-based itaconic acid production.
Article in Biotechnology for biofuels and bioproducts, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- Enhancing acetate-based biosurfactant production by Pseudomonas putida KT2440 through fermentation optimization.Microbial cell factories · 2026Article
- The potential of syngas as a central mediator for a carbon-agnostic circular economy.Nature communications · 2026Review
- Pathway reconstruction of Corynebacterium glutamicum for C30 carotenoid 4,4'-diaponeurosporene production.Journal of biological engineering · 2026Article
- Volatile fatty acids as sustainable precursors for bioproduction: advances in pathway engineering, and bioprocess optimization.World journal of microbiology & biotechnology · 2026Review
- Acetate-containing supernatants from industrial off-gas cultivation enabling high-value product formation with established and emerging production organisms.Biotechnology for biofuels and bioproducts · 2026Article
- A novel engineered strain of Methylorubrum extorquens for methylotrophic production of glycolic acid.Microbial cell factories · 2024Article
- Review of the Proteomics and Metabolic Properties ofMicroorganisms · 2024Review
- Metabolic engineering of Corynebacterium glutamicum for fatty alcohol production from glucose and wheat straw hydrolysate.Biotechnology for biofuels and bioproducts · 2023Article
- Bioupgrading of the aqueous phase of pyrolysis oil from lignocellulosic biomass: a platform for renewable chemicals and fuels from the whole fraction of biomass.Bioresources and bioprocessing · 2023Review
- Improving growth properties of Corynebacterium glutamicum by implementing an iron-responsive protocatechuate biosynthesis.Microbial biotechnology · 2023Article
- High-quality physiology ofFrontiers in bioengineering and biotechnology · 2023Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundItaconic acid is a promising platform chemical for a bio-based polymer industry. Today, itaconic acid is biotechnologically produced with Aspergillus terreus at industrial scale from sugars. The production of fuels but also of chemicals from food substrates is a dilemma since future processes should rely on carbon sources which do not compete for food or feed. Therefore, the production of chemicals from alternative substrates such as acetate is desirable to develop novel value chains in the bioeconomy.
resultsIn this study, Corynebacterium glutamicum ATCC 13032 was engineered to efficiently produce itaconic acid from the non-food substrate acetate. Therefore, we rewired the central carbon and nitrogen metabolism by inactivating the transcriptional regulator RamB, reducing the activity of isocitrate dehydrogenase, deletion of the gdh gene encoding glutamate dehydrogenase and overexpression of cis-aconitate decarboxylase (CAD) from A. terreus optimized for expression in C. glutamicum. The final strain C. glutamicum ΔramB Δgdh IDH
conclusionAs shown in this study, acetate represents a suitable non-food carbon source for itaconic acid production with C. glutamicum. Tailoring the central carbon and nitrogen metabolism enabled the efficient production of itaconic acid from acetate and therefore this study offers useful design principles to genetically engineer C. glutamicum for other products from acetate.
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