Evidence map›Paper›PMID 36515804›Full record

ArticleVirus genes2023

A multiplex real-time RT-PCR assay for the detection of H1, H2 and H3 subtype avian influenza viruses.

Sijing Yan, Fan Yang, Hangping Yao, Dalu Dong, Danna Wu, Nanping Wu, Chunsheng Ye, Haibo Wu

Abstract read
PubMed Publisher
In one paragraph

Article in Virus genes, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Sijing YanState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China.
Fan YangState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China.
Hangping YaoState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China.
Dalu DongHangzhou Biotest Biotech Co.,Ltd, 27 Tuyi Road, Cangqian Street, Yuhang District, Hangzhou, 311121, Zhejiang, China.
Danna WuHangzhou Biotest Biotech Co.,Ltd, 27 Tuyi Road, Cangqian Street, Yuhang District, Hangzhou, 311121, Zhejiang, China.
Nanping WuState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China.
Chunsheng YeHangzhou Biotest Biotech Co.,Ltd, 27 Tuyi Road, Cangqian Street, Yuhang District, Hangzhou, 311121, Zhejiang, China. ethan.ye@biotests.com.cn.
Haibo WuState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China. wuhaibo@zju.edu.cn.
First Affiliated Hospital Zhejiang University · CNHangzhou DAC Biotech (China) · CN

Funding

the Fundamental Research Funds for the Central Universities 2022ZFJH003the Independent Task of State Key Laboratory for Diagnosis and Treatment of Infectious Diseases 2022ZZ02the National Science Foundation of the People's Republic of China 32273092Zhejiang Provincial Natural Science Foundation of China LY19H260006
6 · The paper itself

Abstract

Avian influenza viruses (AIVs) are influenza A viruses, of which subtypes H1, H2 and H3 are highly transmissible in poultry and have the risk of transmission to human as well. It is important to establish an accurate, sensitive and convenient means of virus detection. In this study, we developed a multiplex real-time RT-PCR assay based on conserved sequences of the virus hemagglutinin and matrix, and designed primers and probes for the simultaneous and rapid detection of AIV subtypes H1, H2 and H3. We used different subtypes of AIVs and other avian respiratory viruses for evaluation of the specificity of this method. The results showed good sensitivity, specificity and reproducibility. The detection limit was 10-100 copies per reaction. The method also achieved good concordance with the virus isolation method when compared to 81 poultry samples evaluated. It provides a new method for detecting mixed infections of AIVs.

Indexed as

Influenza A virusInfluenza in BirdsAnimalsHemagglutinin Glycoproteins, Influenza VirusHumansPoultryReproducibility of ResultsReverse Transcriptase Polymerase Chain ReactionSensitivity and SpecificityHemagglutinin Glycoproteins, Influenza VirusAvian influenza virus (AIV)Fluorescent quantitative PCRMultiplex PCRVirus detection

Identifiers

PMID36515804
OpenAlexW4311366963

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.