ArticlePlant physiology2023
Cell-type-specific alternative splicing in the Arabidopsis germline.
Article in Plant physiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- Maintenance of H3K9me2 heterochromatin in the pollen vegetative nucleus requires ARID1 nuclear body formation.The Plant journal : for cell and molecular biology · 2026Article
- Phosphate starvation induces root cell-type-specific transcriptional responses and alternative splicing.The New phytologist · 2026Article
- Occupancy-based mechanism is the chief mode of ROS1 function in preventing DNA hypermethylation.Nature plants · 2026Article
- The Role of Alternative Splicing in Polyploids in Response to Abiotic Stress.International journal of molecular sciences · 2025Review
- Comprehensive mapping of Arabidopsis alternative splicing landscape reveals key insights into plant development and immunity.The plant genome · 2025Article
- Exploring Alternative Splicing in Response to Salinity: A Tissue-Level Comparative Analysis UsingPlants (Basel, Switzerland) · 2025Article
- Camelina circRNA landscape: Implications for gene regulation and fatty acid metabolism.The plant genome · 2025Article
- Bioimaging insights into structural pathways of cell-to-cell communication within the male (MGU) and female (FGU) germ units of Arabidopsis thaliana.Plant cell reports · 2025Article
- Multiple developmental pathways in organisms with developmentally complex life cycles.Frontiers in cell and developmental biology · 2025Review
- The evolutionarily diverged single-stranded DNA-binding proteins SSB1/SSB2 differentially affect the replication, recombination and mutation of organellar genomes inPlant diversity · 2025Article
- ARID1 is required to regulate and reinforce H3K9me2 in sperm cells in Arabidopsis.Nature communications · 2024Article
- Fifty years of sperm cell isolations: from structural to omic studies.Journal of experimental botany · 2023Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During sexual reproduction in flowering plants, the two haploid sperm cells (SCs) embedded within the cytoplasm of a growing pollen tube are carried to the embryo sac for double fertilization. Pollen development in flowering plants is a dynamic process that encompasses changes at transcriptome and epigenome levels. While the transcriptome of pollen and SCs in Arabidopsis (Arabidopsis thaliana) is well documented, previous analyses have mostly been based on gene-level expression. In-depth transcriptome analysis, particularly the extent of alternative splicing (AS) at the resolution of SC and vegetative nucleus (VN), is still lacking. Therefore, we performed RNA-seq analysis to generate a spliceome map of Arabidopsis SCs and VN isolated from mature pollen grains. Based on our de novo transcriptome assembly, we identified 58,039 transcripts, including 9,681 novel transcripts, of which 2,091 were expressed in SCs and 3,600 in VN. Four hundred and sixty-eight genes were regulated both at gene and splicing levels, with many having functions in mRNA splicing, chromatin modification, and protein localization. Moreover, a comparison with egg cell RNA-seq data uncovered sex-specific regulation of transcription and splicing factors. Our study provides insights into a gamete-specific AS landscape at unprecedented resolution.
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