Evidence map›Paper›PMID 36514188›Full record

ArticleCNS neuroscience & therapeutics2023

Downregulation of ADAMTS3 Suppresses Stemness and Tumorigenicity in Glioma Stem Cell.

Hyun-Jin Kim, Hang Yeon Jeong, Don Carlo Batara, Changjong Moon, Seongsoo Lee, Suk Jun Lee, Sang-Ik Park, Moon-Chang Choi, Sung-Hak Kim

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in CNS neuroscience & therapeutics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Hyun-Jin KimDepartment of Animal Science, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Korea.
Hang Yeon JeongDepartment of Animal Science, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Korea.
Don Carlo BataraDepartment of Animal Science, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Korea.
Changjong MoonDepartment of Veterinary Anatomy and Animal Behavior, College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, Gwangju, Korea.
Seongsoo LeeGwangju Center, Korea Basic Science Institute, Gwangju, Korea.
Suk Jun LeeDepartment of Biomedical Laboratory Science, College of Health & Medical Sciences, Cheongju University, Chungbuk, Korea.
Sang-Ik ParkLaboratory of Veterinary Pathology, College of Veterinary Medicine and BK21 Plus Project Team, Chonnam National University, Gwangju, Korea.
Moon-Chang ChoiDepartment of Biomedical Science, Chosun University, Gwangju, Korea.
Sung-Hak KimDepartment of Animal Science, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Korea.ORCID 0000-0001-8970-6648
Chonnam National University · KRCheongju University · KRChosun University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimsGlioblastoma multiforme (GBM) is the most aggressive type of human brain tumor, with a poor prognosis and a median overall survival of fewer than 15 months. Glioma stem cells (GSCs) have recently been identified as a key player in tumor initiation and therapeutic resistance in GBM. ADAMTS family of metalloproteinases is known to cleave a wide range of extracellular matrix substrates and has been linked to tissue remodeling events in tumor development. Here, we investigate that ADAMTS3 regulates GSC proliferation and self-renewal activities, and tumorigenesis in orthotopic xenograft models.

methodsADAMTS3 mRNA expression levels in normal human astrocyte (NHA), glioma, and GSCs cell lines were compared. After knockdown of ADAMTS3, alamarBlue assay, in vitro limiting dilution, and orthotopic xenograft assays were performed. To investigate the tumor-associated roles of ADAMTS3, several statistical assays were conducted using publicly available datasets.

resultsADAMTS3 level was remarkably higher in GSCs than in NHA, glioma cell lines, and their matched differentiated tumor cells. Interestingly, knockdown of ADAMTS3 disrupted GSC's proliferation, self-renewal activity, and tumor formation in vivo. Furthermore, ADAMTS3 could be used as an independent predictor of malignancy progression in GBM.

conclusionWe identified ADAMTS3 as a potential therapeutic target for GBM.

Indexed as

Brain NeoplasmsGlioblastomaGliomaADAMTS ProteinsCell Line, TumorCell ProliferationDown-RegulationHumansNeoplastic Stem CellsProcollagen N-EndopeptidaseADAMTS3 protein, humanADAMTS ProteinsProcollagen N-EndopeptidaseADAMTS3GBMglioma stem celltumor formation

Identifiers

PMID36514188
PMCPMC9873505
OpenAlexW4311327002

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.