Evidence map›Paper›PMID 36513574›Full record

ArticleCytotherapy2023

Impact of storage conditions and duration on function of native and cargo-loaded mesenchymal stromal cell extracellular vesicles.

Daniel Levy, Anjana Jeyaram, Louis J Born, Kai-Hua Chang, Sanaz Nourmohammadi Abadchi, Angela Ting Wei Hsu, Talia Solomon, Amaya Aranda, Samantha Stewart, Xiaoming He and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cytotherapy, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed, 1 pooled it
3.5field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.

  1. Pooled it
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  3. Extension of the fluidics NTF-Biobank of polytraumatized patients with extracellular vesicles: improved vesicle preservation through local processing.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Daniel LevyFischell Department of Bioengineering, University of Maryland, College Park, Maryland, USA.
Anjana JeyaramFischell Department of Bioengineering, University of Maryland, College Park, Maryland, USA.
Louis J BornFischell Department of Bioengineering, University of Maryland, College Park, Maryland, USA.
Kai-Hua ChangDepartment of Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Sanaz Nourmohammadi AbadchiDepartment of Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Angela Ting Wei HsuDepartment of Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Talia SolomonFischell Department of Bioengineering, University of Maryland, College Park, Maryland, USA.
Amaya ArandaFischell Department of Bioengineering, University of Maryland, College Park, Maryland, USA.
Samantha StewartFischell Department of Bioengineering, University of Maryland, College Park, Maryland, USA.
Xiaoming HeFischell Department of Bioengineering, University of Maryland, College Park, Maryland, USA.
John W HarmonDepartment of Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Steven M JayFischell Department of Bioengineering, University of Maryland, College Park, Maryland, USA; Program in Molecular and Cell Biology, University of Maryland, College Park, Maryland, USA. Electronic address: smjay@umd.edu.
University of Maryland, College Park · USJohns Hopkins University · US

Funding

Characterization of the Cardiac Progenitor Cell Exosomes for Optimal TherapeuticsR01HL141922 · NHLBI · UNIVERSITY OF MARYLAND BALTIMORE · PI KAUSHAL, SUNJAY · 2019 to 2022
$2.8M
Dementia Following Spinal Cord Injury: Mechanism and Therapeutic TargetingRF1NS110637 · NINDS · UNIVERSITY OF MARYLAND BALTIMORE · PI JAY, STEVEN MICHAEL, WU, JUNFANG · 2019 to 2019
$2.8M
Training in Host-pathogens interactionsT32AI089621 · NIAID · UNIV OF MARYLAND, COLLEGE PARK · PI LEE, VINCENT T, MCIVER, KEVIN S. · 2010 to 2025
$2.1M
Controlling Exosome Noncoding RNA Cargo for Enhanced Wound HealingR01HL141611 · NHLBI · UNIV OF MARYLAND, COLLEGE PARK · PI JAY, STEVEN MICHAEL · 2018 to 2022
$1.9M
Enabling exosome biomarker development via digitized single vesicle analysisR01GM130923 · NIGMS · UNIV OF MARYLAND, COLLEGE PARK · PI DEVOE, DON L, WHITE, IAN M · 2019 to 2022
$1.7M
Nanohydrocyclones for scalable extracellular vesicle purification and drug loadingR21HL159590 · NHLBI · UNIV OF MARYLAND, COLLEGE PARK · PI DEVOE, DON L, JAY, STEVEN MICHAEL · 2021 to 2022
$421k
NHLBI NIH HHS R01 HL141611NHLBI NIH HHS R01 HL141922NHLBI NIH HHS R21 HL159590NIAID NIH HHS T32 AI089621NIGMS NIH HHS R01 GM130923NINDS NIH HHS RF1 NS110637
6 · The paper itself

Abstract

BACKGROUND

aimsAs evidenced by ongoing clinical trials and increased activity in the commercial sector, extracellular vesicle (EV)-based therapies have begun the transition from bench to bedside. As this progression continues, one critical aspect of EV clinical translation is understanding the effects of storage and transport conditions. Several studies have assessed the impact of storage on EV characteristics such as morphology, uptake and component content, but effects of storage duration and temperature on EV functional bioactivity and, especially, loaded cargo are rarely reported.

methodsThe authors assessed EV outcomes following storage at different temperatures (room temperature, 4°C, -20°C, -80°C) for various durations as well as after lyophilization.

resultsMesenchymal stromal cell (MSC) EVs were observed to retain key aspects of their bioactivity (pro-vascularization, anti-inflammation) for up to 4-6 weeks at -20°C and -80°C and after lyophilization. Furthermore, via in vitro assays and an in vivo wound healing model, these same storage conditions were also demonstrated to enable preservation of the functionality of loaded microRNA and long non-coding RNA cargo in MSC EVs.

conclusionsThese findings extend the current understanding of how EV therapeutic potential is impacted by storage conditions and may inform best practices for handling and storing MSC EVs for both basic research and translational purposes.

Indexed as

Extracellular VesiclesMesenchymal Stem CellsMicroRNAsWound HealingMicroRNAsexosomesHOTAIRmicrovesiclesmiR-146a-5p

Identifiers

PMID36513574
PMCPMC10079553
OpenAlexW4311255691

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.